光漂白后的单光子和双光子荧光恢复
Single- and two-photon fluorescence recovery after photobleaching.
作者信息
Sullivan Kelley D, Majewska Ania K, Brown Edward B
出版信息
Cold Spring Harb Protoc. 2015 Jan 5;2015(1):pdb.top083519. doi: 10.1101/pdb.top083519.
Fluorescence recovery after photobleaching (FRAP) is a microscopy technique for measuring the kinetics of fluorescently labeled molecules and can be applied both in vitro and in vivo for two- and three-dimensional systems. This introduction discusses the three basic FRAP methods: traditional FRAP, multiphoton FRAP (MPFRAP), and FRAP with spatial Fourier analysis (SFA-FRAP). Each discussion is accompanied by a description of the mathematical analysis appropriate for situations in which the recovery kinetics is dictated by free diffusion. In some experiments, the recovery kinetics is dictated by the boundary conditions of the system, and FRAP is then used to quantify the connectivity of various compartments. Because the appropriate mathematical analysis is independent of the bleaching method, the analysis of compartmental connectivity is discussed last, in a separate section.
光漂白后荧光恢复（FRAP）是一种用于测量荧光标记分子动力学的显微镜技术，可应用于体外和体内的二维和三维系统。本介绍讨论了三种基本的FRAP方法：传统FRAP、多光子FRAP（MPFRAP）和具有空间傅里叶分析的FRAP（SFA-FRAP）。每次讨论都伴随着对适合自由扩散决定恢复动力学情况的数学分析的描述。在一些实验中，恢复动力学由系统的边界条件决定，然后使用FRAP来量化各个隔室的连通性。由于适当的数学分析与漂白方法无关，因此在单独的一节中最后讨论隔室连通性的分析。
Zotero 插件