Zhang Zhi-Xiao, Zheng Ying, Hu Ting-Song, Li Qi-Han, Fan Quan-Shui
enter for Disease Control and Prevention, Chengdu Military Region, Kunming, China.
Bing Du Xue Bao. 2014 Sep;30(5):587-93.
Methods for analyses of protein-protein interactions include: yeast two hybrid (Y2H), phage dis- play (PD), co-immunoprecipitation (Co-IP), glutathione S-transferase pull-down (GST pull-down), cellular co-localization, far-western blotting, virus overlay protein binding assay (VOPBA), surface plasmon resonance (SPR), and fluorescence resonance energy transfer (FRET). Technologies for the detection of protein-nucleic acid interactions include: yeast one hybrid (Y1H), chromatin immunoprecipitation (ChIP), electrophoretic mobility shift assay (EMSA), Southwestern blotting, reporter gene, Co-IP, GST pull-down, and PD. These methods are often used in the study of the human enterovirus A71 (EV-A71) by our research team. Reviews in the Chinese literature in this field are lacking, so we reviewed applications of these methods in the study of EV-A71. This review may impart important knowledge in the research of other viruses with regard to protein-protein and protein-nucleic acid interactions.
蛋白质-蛋白质相互作用的分析方法包括：酵母双杂交（Y2H）、噬菌体展示（PD）、免疫共沉淀（Co-IP）、谷胱甘肽S-转移酶下拉实验（GST下拉实验）、细胞共定位、远缘Western印迹法、病毒覆盖蛋白结合分析（VOPBA）、表面等离子体共振（SPR）和荧光共振能量转移（FRET）。蛋白质-核酸相互作用的检测技术包括：酵母单杂交（Y1H）、染色质免疫沉淀（ChIP）、电泳迁移率变动分析（EMSA）、Southwestern印迹法、报告基因、Co-IP、GST下拉实验和PD。我们的研究团队在人类肠道病毒A71（EV-A71）研究中经常使用这些方法。该领域中文文献缺乏综述，因此我们综述了这些方法在EV-A71研究中的应用。本综述可能会为其他病毒在蛋白质-蛋白质和蛋白质-核酸相互作用研究方面提供重要知识。
