评估三种不同浓度的氯己定对人牙本质的持续性。
Evaluation of three different concentrations of Chlorhexidine for their substantivity to human dentin.
作者信息
Singh Harpreet, Kapoor Pooja, Dhillon Jaidev, Kaur Mandeep
机构信息
Department of Conservative Dentistry and Endodontics, Gian Sagar Dental College and Hospital, Patiala, Punjab, India.
Department of Orthodontics and Dentofacial Orthopaedics, Gian Sagar Dental College and Hospital, Patiala, Punjab, India.
出版信息
Indian J Dent. 2014 Oct;5(4):199-201. doi: 10.4103/0975-962X.144726.
AIM
To evaluate the substantivity of different concentrations of Chlorhexidine (CHX) to dentin disks prepared in-vitro.
MATERIALS AND METHODS
Sixty dentin disks were prepared from extracted human third molars and divided into three groups (each containing 20 disks). All the disks were partially demineralized, as per standard procedure. Group A specimens were then treated with 10 microliters of 0.02% Chlorhexidine, Group B specimens with 10 microliters of 0.2% Chlorhexidine, and Group C specimens were treated with 10 microliters of 2% Chlorhexidine. They were then incubated in 1 ml of Phosphatebufferedsaline PBS (pH 7.4). The substantivity was evaluated after 24 hours and one week of incubation. CHX concentration in the eluates was spectrophotometrically analyzed.
RESULTS
A significant amount of CHX was found retained on the dentin disks in Group B as compared to Group C. Also, Group A performed significantly better than group C. However, no statistically significant difference was observed between Group A and Group B.
CONCLUSION
Both 0.02% and 0.2% Chlorhexidine can be clinically recommended when being used for prolonging the durability of resin-dentin bond.
目的
评估不同浓度的洗必泰（CHX）对体外制备的牙本质盘的附着性。
材料与方法
从拔除的人类第三磨牙制备60个牙本质盘，并分为三组（每组含20个牙本质盘）。按照标准程序对所有牙本质盘进行部分脱矿处理。然后，A组样本用10微升0.02%的洗必泰处理，B组样本用10微升0.2%的洗必泰处理，C组样本用10微升2%的洗必泰处理。之后将它们在1毫升磷酸盐缓冲盐水（PBS，pH 7.4）中孵育。在孵育24小时和1周后评估附着性。对洗脱液中的洗必泰浓度进行分光光度分析。
结果
与C组相比，发现B组牙本质盘上保留了大量的洗必泰。此外，A组的表现明显优于C组。然而，A组和B组之间未观察到统计学上的显著差异。
结论
当用于延长树脂 - 牙本质粘结的耐久性时，0.02%和0.2%的洗必泰在临床上均可推荐使用。
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