Hernandez-Molina Gabriela, Burkhardt Amanda M, Lima Guadalupe, Zlotnik Albert, Betanzos Jose L, Bahena Susana, Llorente Luis
Department of Immunology and Rheumatology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga 15. Tlalpan, 14000, Mexico City, D.F., Mexico.
Rheumatol Int. 2015 Aug;35(8):1431-4. doi: 10.1007/s00296-014-3210-0. Epub 2015 Jan 8.
CCL28 is a mucosa-associated epithelial-cell-produced chemokine involved in oral defense. We assessed the level of CCL28 in saliva of primary Sjögren's syndrome (pSS) patients in comparison with healthy controls and correlated it with IgA salivary levels. We included 30 non-smoker pSS patients and 30 non-smoker healthy controls paired by age (±5 years). Saliva samples were collected during the morning and kept frozen at -86 °C until the analysis. Fifty microliters of saliva was diluted 3:1 with water and analyzed for CCL28 salivary levels by ELISA method. The samples were tested in triplicate. IgA salivary levels were tested by ELISA method. We used descriptive statistics, Mann-Whitney U test and Kendall's tau correlation coefficients. pSS patients were mostly females (93.3 %), mean age 54.5 ± 13.3 years and median disease duration of 7.6 years (0.5-33). Patients with pSS had lower levels of salivary CCL28 when compared with controls [0 (0-1,272 pg/ml) vs. 94.4 (0-5,810) pg/ml, p < 0.0001]. pSS patients also had lower median levels of salivary IgA [72.55 μg/ml (0.40-297.4)] than controls [131.9 μg/ml (6.8-281.8)], although the latter results did not reach statistical significance (p = 0.51). Among the SS group, there was no correlation between CCL28 and IgA salivary levels nor between salivary IgA and disease duration, salivary flow, serum immunoglobulins or dental loss. CCL28 was absent in saliva of pSS patients; however, this finding did not correlate with salivary IgA levels.
CCL28是一种与黏膜相关的上皮细胞产生的趋化因子，参与口腔防御。我们评估了原发性干燥综合征（pSS）患者唾液中CCL28的水平，并与健康对照者进行比较，同时将其与唾液中IgA水平相关联。我们纳入了30名非吸烟的pSS患者和30名按年龄匹配（±5岁）的非吸烟健康对照者。在早晨采集唾液样本，并保存在-86°C直至分析。将50微升唾液用水按3:1稀释，并用ELISA法分析唾液中CCL28的水平。样本进行了三次重复检测。唾液中IgA水平采用ELISA法检测。我们使用了描述性统计、曼-惠特尼U检验和肯德尔tau相关系数。pSS患者大多为女性（93.3%），平均年龄54.5±13.3岁，疾病中位病程为7.6年（0.5 - 33年）。与对照组相比，pSS患者唾液中CCL28水平较低[0（0 - 1272 pg/ml）对94.4（0 - 5810）pg/ml，p < 0.0001]。pSS患者唾液中IgA的中位水平[72.55μg/ml（0.40 - 297.4）]也低于对照组[131.9μg/ml（6.8 - 281.8）]，尽管后一结果未达到统计学意义（p = 0.51）。在SS组中，CCL28与唾液中IgA水平之间、唾液中IgA与疾病病程、唾液流量、血清免疫球蛋白或牙齿缺失之间均无相关性。pSS患者唾液中不存在CCL28；然而，这一发现与唾液中IgA水平无关。
