Akbari Hamid, Dalir-Naghadeh Bahram
Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Vet Res Forum. 2014 Winter;5(1):35-41.
To evaluate the effect of intravenous infusion of a soybean based lipid emulsion (Lipovenoes 10%) on some blood constituents in sheep, a replicated 2 × 2 Latin square design experiment was conducted in four clinically healthy ewes. Lipid emulsion (LE group) or normal saline (NS group) was infused intravenously at a rate of 0.025 mL kg(-1) per min for 6 hr and the concentrations of blood triglyceride, glucose, insulin, calcium, magnesium, phosphorous, sodium and potassium were measured before (baseline) and then at timepoints 2, 4, 6, 12 and 24 hr after infusion. Compared to the baseline values and/or NS infusion, LE infusion resulted in a significant increase in the concentrations of triglyceride (p < 0.001), glucose (p < 0.01), calcium (p < 0.05), phosphorous (p < 0.01) and a significant decrease in insulin (p < 0.001) and magnesium (p < 0.05) concentrations. Compared to the baseline value, the homeostasis model of insulin resistance (HOMA-IR) index increased (p < 0.001) at timepoints 2 and 4 hr and abruptly decreased at timepoint six hr (p < 0.01) following LE infusion. In LE group, HOMA-IR values were significantly (p < 0.001) higher than those for NS group at timepoints 2 and 4 hr after infusion. Neither treatment nor time influenced serum sodium and potassium concentrations (p > 0.05). In conclusion, intravenous infusion of Lipovenoes temporarily influenced some blood constituents. Increased triglyceride concentrations were associated with an increase in HOMA-IR values indicating a state of insulin resistance. No remarkable adverse effect was observed following LE infusion and lipid based emulsions can be safely used in ruminants not suffering from extensive lipid mobilization.
为评估静脉输注大豆基脂质乳剂（Lipovenoes 10%）对绵羊某些血液成分的影响，在四只临床健康的母羊中进行了一项重复的2×2拉丁方设计实验。以每分钟0.025 mL kg⁻¹的速率静脉输注脂质乳剂（LE组）或生理盐水（NS组），持续6小时，并在输注前（基线）以及输注后2、4、6、12和24小时测量血液甘油三酯、葡萄糖、胰岛素、钙、镁、磷、钠和钾的浓度。与基线值和/或NS输注相比，LE输注导致甘油三酯（p < 0.001）、葡萄糖（p < 0.01）、钙（p < 0.05）、磷（p < 0.01）浓度显著升高，胰岛素（p < 0.001）和镁（p < 0.05）浓度显著降低。与基线值相比，LE输注后2小时和4小时胰岛素抵抗稳态模型（HOMA-IR）指数升高（p < 0.001），6小时时突然下降（p < 0.01）。在LE组中，输注后2小时和4小时HOMA-IR值显著高于NS组（p < 0.001）。处理和时间均未影响血清钠和钾浓度（p > 0.05）。总之，静脉输注Lipovenoes会暂时影响某些血液成分。甘油三酯浓度升高与HOMA-IR值增加相关，表明存在胰岛素抵抗状态。LE输注后未观察到明显的不良反应，基于脂质的乳剂可安全用于未发生广泛脂质动员的反刍动物。
