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全神经元RNA转运与netrin介导的局部翻译相结合，在空间上调节突触蛋白质组。

Neuron-wide RNA transport combines with netrin-mediated local translation to spatially regulate the synaptic proteome.

作者信息

Kim Sangmok, Martin Kelsey C

机构信息

Department of Biological Chemistry, University of California, Los Angeles, Los Angeles, United States.

出版信息

Elife. 2015 Jan 8;4:e04158. doi: 10.7554/eLife.04158.

DOI:10.7554/eLife.04158
PMID:25569157
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4337609/
Abstract

The persistence of experience-dependent changes in brain connectivity requires RNA localization and protein synthesis. Previous studies have demonstrated a role for local translation in altering the structure and function of synapses during synapse formation and experience-dependent synaptic plasticity. In this study, we ask whether in addition to promoting local translation, local stimulation also triggers directed trafficking of RNAs from nucleus to stimulated synapses. Imaging of RNA localization and translation in cultured Aplysia sensory-motor neurons revealed that RNAs were delivered throughout the arbor of the sensory neuron, but that translation was enriched only at sites of synaptic contact and/or synaptic stimulation. Investigation of the mechanisms that trigger local translation revealed a role for calcium-dependent retrograde netrin-1/DCC receptor signaling. Spatially restricting gene expression by regulating local translation rather than by directing the delivery of mRNAs from nucleus to stimulated synapses maximizes the readiness of the entire neuronal arbor to respond to local cues.

摘要

依赖于经验的大脑连接变化的持续存在需要RNA定位和蛋白质合成。先前的研究已经证明局部翻译在突触形成和依赖于经验的突触可塑性过程中改变突触的结构和功能方面发挥作用。在本研究中，我们探讨除了促进局部翻译外，局部刺激是否还会触发RNA从细胞核向受刺激突触的定向运输。对培养的海兔感觉运动神经元中RNA定位和翻译的成像显示，RNA被输送到感觉神经元的整个树突中，但翻译仅在突触接触和/或突触刺激部位富集。对触发局部翻译机制的研究揭示了钙依赖性逆行网蛋白-1/DCC受体信号传导的作用。通过调节局部翻译而不是通过指导mRNA从细胞核向受刺激突触的运输来在空间上限制基因表达，可使整个神经元树突对局部信号做出反应的准备状态最大化。

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3
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