Wilson Michael S, Nie Weiyan
EIC Laboratories, Inc., 111 Downey Street, Norwood, Massachusetts 02062, USA.
Anal Chem. 2006 Apr 15;78(8):2507-13. doi: 10.1021/ac0518452.
A novel amperometric biosensor for performing simultaneous electrochemical multianalyte immunoassays is described. The sensor consisted of eight iridium oxide sensing electrodes (0.78 mm(2) each), an iridium counter electrode, and a Ag/AgCl reference electrode patterned on a glass substrate. Four different capture antibodies were immobilized on the sensing electrodes via adsorption. Quantification of proteins was achieved using an ELISA in which the electrochemical oxidation of enzyme-generated hydroquinone was measured. The spatial separation of the electrodes enabled simultaneous electrochemical immunoassays for multiple proteins to be conducted in a single assay without amperometric cross-talk between the electrodes. The simultaneous detection of goat IgG, mouse IgG, human IgG, and chicken IgY was demonstrated. The detection limit was 3 ng/mL for all analytes. The sensor had excellent precision (1.9-8.2% interassay CV) and was comparable in performance to commercial single-analyte ELISAs. We anticipate that chip-based sensors, as described herein, will be suitable for the mass production of economical, miniaturized, multianalyte assay devices.
描述了一种用于同时进行电化学多分析物免疫测定的新型安培生物传感器。该传感器由八个氧化铱传感电极（每个0.78平方毫米）、一个铱对电极和一个图案化在玻璃基板上的Ag/AgCl参比电极组成。四种不同的捕获抗体通过吸附固定在传感电极上。使用酶联免疫吸附测定法（ELISA）对蛋白质进行定量，其中测量酶产生的对苯二酚的电化学氧化。电极的空间分离使得能够在单次测定中同时对多种蛋白质进行电化学免疫测定，而电极之间不存在安培串扰。证明了能够同时检测山羊IgG、小鼠IgG、人IgG和鸡IgY。所有分析物的检测限均为3 ng/mL。该传感器具有出色的精密度（批间变异系数为1.9 - 8.2%），性能与商业单分析物ELISA相当。我们预计，本文所述的基于芯片的传感器将适用于经济、小型化多分析物测定设备的大规模生产。
