Lazár Dušan
Department of Biophysics, Center of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 11, 783 71 Olomouc, Czech Republic.
J Plant Physiol. 2015 Mar 1;175:131-47. doi: 10.1016/j.jplph.2014.10.021. Epub 2014 Nov 29.
Almost every laboratory dealing with plant physiology, photosynthesis research, remote sensing, and plant phenotyping possesses a fluorometer to measure a kind of chlorophyll (Chl) fluorescence induction (FLI). When the slow Chl FLI is measured with addition of saturating pulses and far-red illumination, the so-called quenching analysis followed by the so-called relaxation analysis in darkness can be realized. These measurements then serve for evaluation of the so-called energy partitioning, that is, calculation of quantum yields of photochemical and of different types of non-photochemical processes. Several theories have been suggested for photosynthetic energy partitioning. The current work aims to summarize all the existing theories, namely their equations for the quantum yields, their meaning and their assumptions. In the framework of these theories it is also found here that the well-known NPQ parameter ( [Formula: see text] ; Bilger and Björkman, 1990) equals the ratio of the quantum yield of regulatory light-induced non-photochemical quenching to the quantum yield of constitutive non-regulatory non-photochemical quenching (ΦNPQ/Φf,D). A similar relationship is also found here for the PQ parameter (ΦP/Φf,D).
几乎每个从事植物生理学、光合作用研究、遥感和植物表型分析的实验室都拥有一台用于测量某种叶绿素（Chl）荧光诱导（FLI）的荧光计。当通过添加饱和脉冲和远红光照射来测量缓慢的Chl FLI时，可以实现所谓的猝灭分析，随后在黑暗中进行所谓的弛豫分析。这些测量随后用于评估所谓的能量分配，即计算光化学和不同类型非光化学过程的量子产率。已经提出了几种关于光合能量分配的理论。当前的工作旨在总结所有现有的理论，即它们的量子产率方程、含义和假设。在这些理论的框架内，还发现这里著名的NPQ参数（[公式：见正文]；Bilger和Björkman，1990）等于调节性光诱导非光化学猝灭的量子产率与组成性非调节性非光化学猝灭的量子产率之比（ΦNPQ/Φf,D）。这里还发现PQ参数（ΦP/Φf,D）也有类似的关系。
