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基于多重熔解分析的DNA快速超声等温扩增——在性传播疾病临床诊断中的应用

Rapid ultrasonic isothermal amplification of DNA with multiplexed melting analysis – applications in the clinical diagnosis of sexually transmitted diseases.

作者信息

Xu Gaolian, Gunson Rory N, Cooper Jonathan M, Reboud Julien

机构信息

Division of Biomedical Engineering, School of Engineering, University of Glasgow, Oakfield Avenue, Rankine Building, G12 8LT Glasgow, UK.

出版信息

Chem Commun (Camb). 2015 Feb 14;51(13):2589-92. doi: 10.1039/c4cc08389j.

DOI:10.1039/c4cc08389j
PMID:25569801
Abstract

We describe a nucleic acid testing (NAT) platform for infectious disease diagnostics at the point-of-care, using surface acoustic waves (SAW) to perform a multiplexed loop-mediated isothermal amplification (LAMP) test for sexually transmitted diseases. The ultrasonic actuation not only enables faster NAT reactions but also provides a route towards integrating low-cost, low-power molecular diagnostics into disposable sensors.

摘要

我们描述了一种用于即时医疗传染病诊断的核酸检测（NAT）平台，该平台利用表面声波（SAW）对性传播疾病进行多重环介导等温扩增（LAMP）检测。超声驱动不仅能加快核酸检测反应，还为将低成本、低功耗分子诊断集成到一次性传感器中提供了一条途径。

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