School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu 211166, China.
Department of Mechanical Engineering and Material Science, Duke University, Durham, North Carolina 27708, United States.
ACS Nano. 2020 Mar 24;14(3):3159-3169. doi: 10.1021/acsnano.9b08349. Epub 2020 Mar 2.
Acoustofluidic methods, with advantages including simplicity of device design, biocompatible manipulation, and low power consumption, have been touted as promising tools for point-of-care (POC) testing. Here, we report a cell-phone-based acoustofluidic platform that uses acoustic radiation forces to enrich nanoscale analytes and red and green fluorescence nanoparticles (SiO@R and G@SiO) as probes for POC visual testing. Thus, the color signals from the fluorescent probes are enhanced, and colorimetric sensitivity is significantly improved. As a POC demonstration, the acoustofluidic platform is used to detect hemoglobin (Hb) from human blood, resulting in a rapid and straightforward measurement of normal blood Hb levels. Combining an acoustofluidic-based nanoparticle-concentration platform with cell-phone-based colorimetry, our method introduces a potential pathway toward practical POC testing.
声流方法具有设备设计简单、生物相容性好、功耗低等优点，被认为是即时检测（POC）的有前途的工具。在这里，我们报告了一种基于手机的声流平台，该平台利用声辐射力来富集纳米级分析物，以及红色和绿色荧光纳米颗粒（SiO@R 和 G@SiO）作为 POC 视觉检测的探针。因此，荧光探针的颜色信号得到增强，比色灵敏度得到显著提高。作为 POC 的演示，该声流平台用于检测人血中的血红蛋白（Hb），从而快速直接地测量正常血液 Hb 水平。将基于声流的纳米粒子浓缩平台与基于手机的比色法相结合，我们的方法为实际的 POC 测试引入了一种潜在的途径。
