Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, PR China.
Biosens Bioelectron. 2015 Jun 15;68:168-174. doi: 10.1016/j.bios.2014.12.046. Epub 2014 Dec 23.
This paper reports a novel nanosensor for organophosphorus pesticides based on the fluorescence resonance energy transfer (FRET) between NaYF4:Yb,Er upconversion nanoparticles (UCNPs) and gold nanoparticles (AuNPs). The detection mechanism is based on the facts that AuNPs quench the fluorescence of UCNPs and organophosphorus pesticides (OPs) inhibit the activity of acetylcholinesterase (AChE) which catalyzes the hydrolysis of acetylthiocholine (ATC) into thiocholine. Under the optimized conditions, the logarithm of the pesticides concentration was proportional to the inhibition efficiency. The detection limits of parathion-methyl, monocrotophos and dimethoate reached 0.67, 23, and 67 ng/L, respectively. Meanwhile, the biosensor shows good sensitivity, stability, and could be successfully applied to detection of OPs in real food samples, suggesting the biosensor has potentially extensive application clinic diagnoses assays.
本文报道了一种基于上转换纳米粒子（UCNPs）与金纳米粒子（AuNPs）之间荧光共振能量转移（FRET）的新型有机磷农药纳米传感器。检测机制基于以下事实：AuNPs 猝灭 UCNPs 的荧光，而有机磷农药（OPs）抑制乙酰胆碱酯酶（AChE）的活性，AChE 催化乙酰硫代胆碱（ATC）水解为硫代胆碱。在优化条件下，农药浓度的对数与抑制效率成正比。对甲基对硫磷、久效磷和乐果的检测限分别达到 0.67、23 和 67ng/L。同时，该生物传感器具有良好的灵敏度、稳定性，可成功应用于实际食品样品中 OPs 的检测，表明该生物传感器具有广泛的临床诊断应用潜力。
