Chen Chong, Lei Hong, Liu Nan, Yan Hui
School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China.
Food Chem X. 2022 Sep 6;15:100439. doi: 10.1016/j.fochx.2022.100439. eCollection 2022 Oct 30.
This paper reports a portable fluorescence resonance energy transfer (FRET) aptasensor for ampicillin (Amp) detection using upconversion particles (UCNPs) as energy donors and Au nanoparticles (AuNPs) as energy acceptors. The optimal parameters of the detection system were investigated. Under the optimal conditions, it had a good linear relationship between the fluorescence intensities and Amp concentrations, a high coefficient of determination (R) of 0.9939, a wide detection range of 10-100 ng/mL, and a low limit of detection (LOD) of 3.9 ng/mL; meanwhile, the aptasensor had high selectivity for Amp against the interference of other antibiotics, and had good recovery and repeatability. Also, its detection performance had been successfully validated by milk samples. Therefore, the developed aptasensor based on FRET between UCNPs and AuNPs has a good prospect for Amp on-site detection in milk with a portable upconversion detection instrument.
本文报道了一种便携式荧光共振能量转移（FRET）适体传感器，用于检测氨苄青霉素（Amp），该传感器以上转换纳米颗粒（UCNPs）作为能量供体，金纳米颗粒（AuNPs）作为能量受体。研究了检测系统的最佳参数。在最佳条件下，荧光强度与Amp浓度之间具有良好的线性关系，决定系数（R）高达0.9939，检测范围宽，为10 - 100 ng/mL，检测限低，为3.9 ng/mL；同时，该适体传感器对Amp具有高选择性，可抵抗其他抗生素的干扰，并且具有良好的回收率和重复性。此外，其检测性能已通过牛奶样品成功验证。因此，基于UCNPs与AuNPs之间FRET开发的适体传感器，利用便携式上转换检测仪器对牛奶中的Amp进行现场检测具有良好的前景。
