上转换发光法同时检测人血清中的过氧化氢和葡萄糖。
Simultaneous detection of hydrogen peroxide and glucose in human serum with upconversion luminescence.
机构信息
State Key Laboratory of Chemical Resource Engineering, Beijing Key Laboratory of Environmentally Harmful Chemical Analysis, Beijing University of Chemical Technology, Beijing 100029, PR China.
Department of Gastroenterology, the Military General Hospital of Beijing PLA, PR China.
出版信息
Biosens Bioelectron. 2015 Jun 15;68:204-209. doi: 10.1016/j.bios.2014.12.053. Epub 2014 Dec 24.
In this paper, a green upconversion photoluminescence (UCPL) system for the highly sensitive and selective detection of H2O2 and glucose in human sera was developed by utilizing the excellent optical properties of NaYF4:Yb(3+)/Er(3+) upconversion nanoparticles (UCNPs). In the presence of H2O2, the colorless 3,3',5,5'-tetramethylbenzidine (TMB) was oxidized into blue oxidized TMB (oxTMB) by assistance of horseradish peroxidase (HRP). And the green UCPL of UCNPs was quenched by the oxTMB linearly over the range of 100 nM-4.0 μM with a low limit of detection (LOD) of 45.0 nM for H2O2. Based on the transformation of glucose into H2O2 by means of glucose oxidase, the TMB-UCNPs-HRP system was further exploited for the highly sensitive detection of glucose in the range of 0.1-5.0 μM with a LOD of 64.0 nM in human sera independent of preconcentration and purification. The results are in good agreement with the clinical data, suggesting that this UCPL nanosensor is highly practical.
本文利用 NaYF4:Yb(3+)/Er(3+) 上转换纳米粒子（UCNPs）的优异光学性质，开发了一种用于高灵敏度和选择性检测人血清中 H2O2 和葡萄糖的绿色上转换光致发光（UCPL）系统。在 H2O2 的存在下，无色 3,3',5,5'-四甲基联苯胺（TMB）在辣根过氧化物酶（HRP）的辅助下被氧化成蓝色氧化 TMB（oxTMB）。UCNPs 的绿色 UCPL 被 oxTMB 线性猝灭，其对 H2O2 的检测下限（LOD）低至 45.0 nM。基于葡萄糖氧化酶将葡萄糖转化为 H2O2，TMB-UCNPs-HRP 系统进一步用于人血清中葡萄糖的高灵敏度检测，在 0.1-5.0 μM 范围内的检测下限（LOD）为 64.0 nM，无需预浓缩和纯化。结果与临床数据吻合良好，表明该 UCPL 纳米传感器具有很高的实用性。
Zotero 插件