Pires N M M, Dong T
Annu Int Conf IEEE Eng Med Biol Soc. 2014;2014:4411-4. doi: 10.1109/EMBC.2014.6944602.
This work reports on integrated passive-flow optical microfluidic devices to detect waterborne pathogens in the field. Ring-shaped organic photodiodes were integrated to a capillary-induced flow microfluidic channel for monitoring chemiluminescent sandwich immunoassays enhanced by gold nanoparticles. The integrated device yielded a resolution of 4×10(4) cells/mL for the detection of Legionella pneumophila, which represented a 25-fold improvement over chemiluminescence detection devices employing no gold-nanoparticle enhancement. This work demonstrates the feasibility of a low-cost but highly sensitive lab-on-a-chip device amenable for point-of-use applications.
这项工作报道了用于现场检测水源性病原体的集成无源流动光学微流控装置。将环形有机光电二极管集成到毛细管诱导流动微流控通道中，用于监测由金纳米颗粒增强的化学发光夹心免疫分析。该集成装置对嗜肺军团菌的检测分辨率为4×10⁴ 个细胞/毫升，与未采用金纳米颗粒增强的化学发光检测装置相比，提高了25倍。这项工作证明了一种低成本但高灵敏度的芯片实验室装置用于现场即时检测应用的可行性。
