沼蛙线粒体基因组的完整核苷酸序列及基因重排
Complete nucleotide sequence and gene rearrangement of the mitochondrial genome of Occidozyga martensii.
作者信息
Li En, Li Xiaoqiang, Wu Xiaobing, Feng Ge, Zhang Man, Shi Haitao, Wang Lijun, Jiang Jianping
机构信息
College of Life Sciences, Anhui Province Key Laboratory for Conservation and Exploitation of Biological Resource, Anhui Normal University, Wuhu 241000, People's Republic of China.
出版信息
J Genet. 2014 Dec;93(3):631-41. doi: 10.1007/s12041-014-0418-4.
In this study, the complete nucleotide sequence (18,321 bp) of the mitochondrial (mt) genome of the round-tongued floating frog, Occidozyga martensii was determined. Although, the base composition and codon usage of O. martensii conformed to the typical vertebrate patterns, this mt genome contained 23 tRNAs (a tandem duplication of tRNA-Met gene). The LTPF tRNA-gene cluster, and the derived position of the ND5 gene downstream of the control region, were present in this mitogenome. Moreover, we found that in the WANCY tRNA-gene cluster, the tRNA-Asn gene was located between the tRNATyr and COI genes instead of between the tRNA-Ala and tRNA-Cys genes, which is a novel mtDNA gene rearrangement in vertebrates. Based on the concatenated nucleotide sequences of the 13 protein-coding genes, phylogenetic analysis (BI, ML, MP) was performed to further clarify the phylogenetic relations of this species within anurans.
在本研究中，测定了圆舌浮蛙（Occidozyga martensii）线粒体（mt）基因组的完整核苷酸序列（18,321 bp）。尽管圆舌浮蛙的碱基组成和密码子使用符合典型的脊椎动物模式，但该线粒体基因组包含23个tRNA（tRNA-Met基因的串联重复）。该有丝分裂基因组中存在LTPF tRNA基因簇以及控制区下游ND5基因的衍生位置。此外，我们发现，在WANCY tRNA基因簇中，tRNA-Asn基因位于tRNATyr和COI基因之间，而非tRNA-Ala和tRNA-Cys基因之间，这是脊椎动物中一种新的线粒体DNA基因重排。基于13个蛋白质编码基因的串联核苷酸序列，进行了系统发育分析（贝叶斯推断、最大似然法、最大简约法），以进一步阐明该物种在无尾目中的系统发育关系。
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