Jiang Lichun, Zhang Min, Deng Lu, Xu Zhongwen, Shi Hongyan, Jia Xiaodong, Lai Zhenli, Ruan Qiping, Chen Wei
Key Laboratory for Molecular Biology and Biopharmaceutics School of Life Science and Technology Mianyang Normal University Mianyang China.
Ecological Security and Protection Key Laboratory of Sichuan Province Mianyang Normal University Mianyang China.
Ecol Evol. 2020 Oct 31;10(23):12817-12837. doi: 10.1002/ece3.6824. eCollection 2020 Dec.
The Omei wood frog (), endemic to central China, belongs to the family Ranidae. In this study, we achieved detail knowledge about the mitogenome of the species. The length of the genome is 20,120 bp, including 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and a noncoding control region. Similar to other amphibians, we found that only nine genes (ND6 and eight tRNA genes) are encoded on the light strand (L) and other genes on the heavy strand (H). Totally, The base composition of the mitochondrial genome included 27.29% A, 28.85% T, 28.87% C, and 15.00% G, respectively. The control regions among the species were found to exhibit rich genetic variability and A + T content. was clustered together with in phylogenetic tree. Compared to and , it was more closely related to , and a new way of gene rearrangement (ND6-trnE-Cytb-D-loop-trnL2 (CUN)-ND5-D-loop) was also found in the mitogenome of and . Our results about the mitochondrial genome of will contribute to the future studies on phylogenetic relationship and the taxonomic status of and related Ranidae species.
峨眉林蛙（），原产于中国中部，属于蛙科。在本研究中，我们获得了该物种线粒体基因组的详细信息。基因组长度为20,120碱基对，包括13个蛋白质编码基因（PCGs）、22个tRNA基因、2个rRNA基因和一个非编码控制区。与其他两栖动物类似，我们发现只有9个基因（ND6和8个tRNA基因）在轻链（L）上编码，其他基因在重链（H）上编码。线粒体基因组的碱基组成分别为27.29%A、28.85%T、28.87%C和15.00%G。发现该物种间的控制区表现出丰富的遗传变异性和A+T含量。在系统发育树中与聚在一起。与和相比，它与的关系更密切，并且在和的线粒体基因组中还发现了一种新的基因重排方式（ND6-trnE-Cytb-D-loop-trnL2（CUN）-ND5-D-loop）。我们关于线粒体基因组的研究结果将有助于未来对及其相关蛙科物种的系统发育关系和分类地位的研究。
