Morgado Sergio Mascarenhas, Vicente Ana Carolina Paulo
Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Genética Molecular de Microrganismos, Rio de Janeiro, RJ, Brasil.
Mem Inst Oswaldo Cruz. 2019 Jan 7;114:e180348. doi: 10.1590/0074-02760180348.
Shared traits between prokaryotes and eukaryotes are helpful in the understanding of the tree of life evolution. In bacteria and eukaryotes, it has been shown a particular organisation of tRNA genes as clusters, but this trait has not been explored in the archaea domain.
Explore the occurrence of tRNA gene clusters in archaea.
In-silico analyses of complete and draft archaeal genomes based on tRNA gene isotype and synteny, tRNA gene cluster content and mobilome elements.
We demonstrated the prevalence of tRNA gene clusters in archaea. tRNA gene clusters, composed of archaeal-type tRNAs, were identified in two Archaea class, Halobacteria and Methanobacteria from Euryarchaeota supergroup. Genomic analyses also revealed evidence of the association between tRNA gene clusters to mobile genetic elements and intra-domain horizontal gene transfer.
tRNA gene cluster occurs in the three domains of life, suggesting a role of this type of tRNA gene organisation in the biology of the living organisms.
原核生物和真核生物之间的共同特征有助于理解生命之树的进化。在细菌和真核生物中，已显示出tRNA基因作为簇的特定组织形式，但在古菌域中尚未对这一特征进行探索。
探索古菌中tRNA基因簇的存在情况。
基于tRNA基因同型和同线性、tRNA基因簇含量以及可移动基因组元件，对完整和草图古菌基因组进行电子分析。
我们证明了tRNA基因簇在古菌中的普遍性。在广古菌超群的两个古菌类群，即嗜盐菌和甲烷菌中，鉴定出了由古菌型tRNA组成的tRNA基因簇。基因组分析还揭示了tRNA基因簇与可移动遗传元件以及域内水平基因转移之间存在关联的证据。
tRNA基因簇存在于生命的三个域中，表明这种类型的tRNA基因组织在生物体生物学中具有一定作用。
