通过将多聚组氨酸标记的膜联蛋白V与纳米颗粒药物偶联制备的细胞保护纳米颗粒。
Cytoprotective nanoparticles by conjugation of a polyhis tagged annexin V to a nanoparticle drug.
作者信息
Chen Howard H, Yuan Hushan, Cho Hoonsung, Sosnovik David E, Josephson Lee
机构信息
Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Boston, MA 02129, USA.
出版信息
Nanoscale. 2015 Feb 14;7(6):2255-9. doi: 10.1039/c4nr06861k.
We synthesized a cytoprotective magnetic nanoparticle by reacting a maleimide functionalized Feraheme (FH) with a disulfide linked dimer of a polyhis tagged annexin V. Following reductive cleavage of disulfide, the resulting annexin-nanoparticle (diameter = 28.0 ± 2.0 nm by laser light scattering, 7.6 annexin's/nanoparticle) was cytoprotective to cells subjected to plasma membrane disrupting chemotherapeutic or mechanical stresses, and significantly more protective than the starting annexin V. Annexin-nanoparticles provide an approach to the design of nanomaterials which antagonize the plasma membrane permeability characteristic of necrosis and which may have applications as cytoprotective agents.
我们通过使马来酰亚胺功能化的血红素铁（FH）与聚组氨酸标记的膜联蛋白V的二硫键连接二聚体反应，合成了一种具有细胞保护作用的磁性纳米颗粒。二硫键经还原裂解后，所得的膜联蛋白 - 纳米颗粒（通过激光散射法测得直径为28.0 ± 2.0 nm，每个纳米颗粒含7.6个膜联蛋白）对遭受质膜破坏化疗或机械应激的细胞具有细胞保护作用，且比起始的膜联蛋白V具有显著更强的保护作用。膜联蛋白 - 纳米颗粒为纳米材料的设计提供了一种方法，这种纳米材料可对抗坏死的质膜通透性特征，并可能作为细胞保护剂应用。
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