• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

含恶唑酮碱基对的稳定性对DNA延伸的影响。

Effects of stability of base pairs containing an oxazolone on DNA elongation.

作者信息

Suzuki Masayo, Ohtsuki Kazuya, Kino Katsuhito, Kobayashi Teruhiko, Morikawa Masayuki, Kobayashi Takanobu, Miyazawa Hiroshi

机构信息

Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, 1314-1 Shido, Sanuki, Kagawa 769-2193, Japan.

出版信息

J Nucleic Acids. 2014;2014:178350. doi: 10.1155/2014/178350. Epub 2014 Dec 7.

DOI:10.1155/2014/178350
PMID:25574383
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4273591/
Abstract

The nucleoside 2,2,4-triamino-5(2H)-oxazolone (Oz) can result from oxidative damage to guanine residues in DNA. Despite differences among the three polymerases (Pol β, KF exo(-), and Pol η) regarding nucleotide incorporation patterns opposite Oz, all three polymerases can incorporate guanine opposite Oz. Based on ab initio calculations, we proposed a structure for a stable Oz:G base pair. Here, to assess the stability of each Oz-containing base pair (Oz:G, Oz:A, Oz:C, and Oz:T) upon DNA replication, we determined the efficiency of Pol β-, KF exo(-)-, or Pol η-catalyzed primer extension beyond each base pair. With each polymerase, extension beyond Oz:G was more efficient than that beyond Oz:A, Oz:C, or Oz:T. Moreover, thermal denaturation studies revealed that the T m value for the duplex containing Oz:G was significantly higher than those obtained for duplexes containing Oz:A, Oz:C, or Oz:T. Therefore, the results from ab initio calculations along with those from DNA replication assays and thermal denaturation experiments supported the conclusion that Oz:G is the most stable of the Oz-containing base pairs.

摘要

核苷2,2,4-三氨基-5(2H)-恶唑酮（Oz）可由DNA中鸟嘌呤残基的氧化损伤产生。尽管三种聚合酶（Pol β、KF exo(-)和Pol η）在与Oz相对的核苷酸掺入模式上存在差异，但这三种聚合酶都能在Oz相对位置掺入鸟嘌呤。基于从头算计算，我们提出了一种稳定的Oz:G碱基对结构。在此，为了评估每个含Oz碱基对（Oz:G、Oz:A、Oz:C和Oz:T）在DNA复制时的稳定性，我们测定了Pol β、KF exo(-)或Pol η催化的引物延伸越过每个碱基对的效率。对于每种聚合酶，越过Oz:G的延伸比越过Oz:A、Oz:C或Oz:T的延伸更有效。此外，热变性研究表明，含Oz:G的双链体的熔解温度（Tm）值显著高于含Oz:A、Oz:C或Oz:T的双链体的Tm值。因此，从头算计算结果以及DNA复制测定和热变性实验结果支持了Oz:G是含Oz碱基对中最稳定的这一结论。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e7a/4273591/0331ec1cd58b/JNA2014-178350.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e7a/4273591/d00df3e8a51c/JNA2014-178350.sch.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e7a/4273591/382c3c8d6662/JNA2014-178350.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e7a/4273591/4307b405ea28/JNA2014-178350.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e7a/4273591/66dd78378a3d/JNA2014-178350.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e7a/4273591/86074708bab0/JNA2014-178350.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e7a/4273591/4b8ff0a26439/JNA2014-178350.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e7a/4273591/0331ec1cd58b/JNA2014-178350.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e7a/4273591/d00df3e8a51c/JNA2014-178350.sch.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e7a/4273591/382c3c8d6662/JNA2014-178350.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e7a/4273591/4307b405ea28/JNA2014-178350.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e7a/4273591/66dd78378a3d/JNA2014-178350.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e7a/4273591/86074708bab0/JNA2014-178350.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e7a/4273591/4b8ff0a26439/JNA2014-178350.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0e7a/4273591/0331ec1cd58b/JNA2014-178350.006.jpg

相似文献

1
Effects of stability of base pairs containing an oxazolone on DNA elongation.含恶唑酮碱基对的稳定性对DNA延伸的影响。
J Nucleic Acids. 2014;2014:178350. doi: 10.1155/2014/178350. Epub 2014 Dec 7.
2
Contiguous 2,2,4-triamino-5(2H)-oxazolone obstructs DNA synthesis by DNA polymerases α, β, η, ι, κ, REV1 and Klenow Fragment exo-, but not by DNA polymerase ζ.相邻的2,2,4-三氨基-5（2H）-恶唑酮可阻碍DNA聚合酶α、β、η、ι、κ、REV1和Klenow片段外切酶进行DNA合成，但不会阻碍DNA聚合酶ζ。
J Biochem. 2016 Mar;159(3):323-9. doi: 10.1093/jb/mvv103. Epub 2015 Oct 21.
3
Analysis of nucleotide insertion opposite 2,2,4-triamino-5(2H)-oxazolone by eukaryotic B- and Y-family DNA polymerases.真核生物B族和Y族DNA聚合酶对2,2,4-三氨基-5(2H)-恶唑酮对面核苷酸插入的分析。
Chem Res Toxicol. 2015 Jun 15;28(6):1307-16. doi: 10.1021/acs.chemrestox.5b00114. Epub 2015 Jun 3.
4
Calculating distortions of short DNA duplexes with base pairing between an oxidatively damaged guanine and a guanine.计算氧化损伤的鸟嘌呤与鸟嘌呤之间碱基配对的短DNA双链体的扭曲情况。
Molecules. 2014 Jul 28;19(8):11030-44. doi: 10.3390/molecules190811030.
5
Generation, repair and replication of guanine oxidation products.鸟嘌呤氧化产物的生成、修复与复制。
Genes Environ. 2017 Aug 1;39:21. doi: 10.1186/s41021-017-0081-0. eCollection 2017.
6
A DNA oligomer containing 2,2,4-triamino-5(2H)-oxazolone is incised by human NEIL1 and NTH1.一种含有 2,2,4-三氨基-5(2H)-恶唑啉的 DNA 寡聚体可被人源 NEIL1 和 NTH1 切割。
Mutat Res. 2012 Jun 1;734(1-2):73-7. doi: 10.1016/j.mrfmmm.2012.03.007. Epub 2012 Mar 23.
7
Analysis of nucleotide insertion and extension at 8-oxo-7,8-dihydroguanine by replicative T7 polymerase exo- and human immunodeficiency virus-1 reverse transcriptase using steady-state and pre-steady-state kinetics.利用稳态和预稳态动力学，通过复制性T7聚合酶外切酶和人类免疫缺陷病毒1逆转录酶分析8-氧代-7,8-二氢鸟嘌呤处的核苷酸插入和延伸情况。
Biochemistry. 1997 May 27;36(21):6475-87. doi: 10.1021/bi9627267.
8
Steady-state and pre-steady-state kinetic analysis of dNTP insertion opposite 8-oxo-7,8-dihydroguanine by Escherichia coli polymerases I exo- and II exo-.大肠杆菌聚合酶 I 外切酶和 II 外切酶对与 8-氧代-7,8-二氢鸟嘌呤相对的脱氧核苷三磷酸插入的稳态和预稳态动力学分析。
Biochemistry. 1996 Jul 30;35(30):9840-9. doi: 10.1021/bi960485x.
9
Calculation of the stabilization energies of oxidatively damaged guanine base pairs with guanine.氧化损伤的鸟嘌呤碱基对与鸟嘌呤的稳定能计算。
Molecules. 2012 Jun 1;17(6):6705-15. doi: 10.3390/molecules17066705.
10
Products of Oxidative Guanine Damage Form Base Pairs with Guanine.氧化鸟嘌呤损伤产物与鸟嘌呤形成碱基对。
Int J Mol Sci. 2020 Oct 15;21(20):7645. doi: 10.3390/ijms21207645.

引用本文的文献

1
Analysis of nucleotide insertion opposite urea and translesion synthesis across urea by DNA polymerases.DNA聚合酶对尿素的核苷酸插入及跨尿素的跨损伤合成分析。
Genes Environ. 2022 Feb 15;44(1):7. doi: 10.1186/s41021-022-00236-3.
2
Products of Oxidative Guanine Damage Form Base Pairs with Guanine.氧化鸟嘌呤损伤产物与鸟嘌呤形成碱基对。
Int J Mol Sci. 2020 Oct 15;21(20):7645. doi: 10.3390/ijms21207645.
3
Generation, repair and replication of guanine oxidation products.鸟嘌呤氧化产物的生成、修复与复制。

本文引用的文献

1
Calculating distortions of short DNA duplexes with base pairing between an oxidatively damaged guanine and a guanine.计算氧化损伤的鸟嘌呤与鸟嘌呤之间碱基配对的短DNA双链体的扭曲情况。
Molecules. 2014 Jul 28;19(8):11030-44. doi: 10.3390/molecules190811030.
2
Calculation of the stabilization energies of oxidatively damaged guanine base pairs with guanine.氧化损伤的鸟嘌呤碱基对与鸟嘌呤的稳定能计算。
Molecules. 2012 Jun 1;17(6):6705-15. doi: 10.3390/molecules17066705.
3
Analysis of mice deficient in both REV1 catalytic activity and POLH reveals an unexpected role for POLH in the generation of C to G and G to C transversions during Ig gene hypermutation.
Genes Environ. 2017 Aug 1;39:21. doi: 10.1186/s41021-017-0081-0. eCollection 2017.
4
Excessive Reactive Oxygen Species and Exotic DNA Lesions as an Exploitable Liability.过量活性氧物种和外来DNA损伤作为一种可利用的不利因素。
Biochemistry. 2016 Sep 27;55(38):5341-52. doi: 10.1021/acs.biochem.6b00703. Epub 2016 Sep 13.
5
Contiguous 2,2,4-triamino-5(2H)-oxazolone obstructs DNA synthesis by DNA polymerases α, β, η, ι, κ, REV1 and Klenow Fragment exo-, but not by DNA polymerase ζ.相邻的2,2,4-三氨基-5（2H）-恶唑酮可阻碍DNA聚合酶α、β、η、ι、κ、REV1和Klenow片段外切酶进行DNA合成，但不会阻碍DNA聚合酶ζ。
J Biochem. 2016 Mar;159(3):323-9. doi: 10.1093/jb/mvv103. Epub 2015 Oct 21.
分析同时缺乏 REV1 催化活性和 POLH 的小鼠表明，POLH 在 Ig 基因超突变过程中产生 C 到 G 和 G 到 C 颠换中具有意想不到的作用。
Int Immunol. 2012 Mar;24(3):169-74. doi: 10.1093/intimm/dxr109. Epub 2012 Jan 5.
4
Eukaryotic DNA polymerases alpha, beta and epsilon incorporate guanine opposite 2,2,4-triamino-5(2H)-oxazolone.真核生物DNA聚合酶α、β和ε能将鸟嘌呤掺入到与2,2,4-三氨基-5（2H）-恶唑酮相对的位置。
Chembiochem. 2009 Nov 2;10(16):2613-6. doi: 10.1002/cbic.200900492.
5
Translesion synthesis by human DNA polymerase eta across oxidative products of guanine.人DNA聚合酶η跨鸟嘌呤氧化产物进行的跨损伤合成。
Nucleic Acids Symp Ser (Oxf). 2004(48):171-2. doi: 10.1093/nass/48.1.171.
6
Quantitative analysis of the oxidative DNA lesion, 2,2-diamino-4-(2-deoxy-beta-D-erythro-pentofuranosyl)amino]-5(2H)-oxazolone (oxazolone), in vitro and in vivo by isotope dilution-capillary HPLC-ESI-MS/MS.通过同位素稀释-毛细管高效液相色谱-电喷雾串联质谱法对氧化DNA损伤产物2,2-二氨基-4-（2-脱氧-β-D-赤型-呋喃戊糖基）氨基]-5（2H）-恶唑酮（恶唑酮）进行体内外定量分析。
Nucleic Acids Res. 2006;34(19):5449-60. doi: 10.1093/nar/gkl596. Epub 2006 Oct 4.
7
Influence of DNA structure on DNA polymerase beta active site function: extension of mutagenic DNA intermediates.DNA结构对DNA聚合酶β活性位点功能的影响：诱变DNA中间体的延伸
J Biol Chem. 2004 Jul 23;279(30):31921-9. doi: 10.1074/jbc.M404016200. Epub 2004 May 15.
8
Local deformations revealed by dynamics simulations of DNA polymerase Beta with DNA mismatches at the primer terminus.通过对引物末端存在DNA错配的DNA聚合酶β进行动力学模拟揭示的局部变形。
J Mol Biol. 2002 Aug 16;321(3):459-78. doi: 10.1016/s0022-2836(02)00617-4.
9
Error rate and specificity of human and murine DNA polymerase eta.人类和小鼠DNA聚合酶η的错误率和特异性
J Mol Biol. 2001 Sep 14;312(2):335-46. doi: 10.1006/jmbi.2001.4937.
10
The pH-dependent role of superoxide in riboflavin-catalyzed photooxidation of 8-oxo-7,8-dihydroguanosine.超氧化物在核黄素催化的8-氧代-7,8-二氢鸟苷光氧化反应中pH依赖性作用
Org Lett. 2001 Sep 6;3(18):2801-4. doi: 10.1021/ol0161763.