DNA聚合酶对尿素的核苷酸插入及跨尿素的跨损伤合成分析。
Analysis of nucleotide insertion opposite urea and translesion synthesis across urea by DNA polymerases.
作者信息
Kawada Taishu, Kino Katsuhito, Tokorodani Kyousuke, Anabuki Ryuto, Morikawa Masayuki, Kobayashi Takanobu, Ohara Kazuaki, Ohshima Takayuki, Miyazawa Hiroshi
机构信息
Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, 1314-1 Shido, Sanuki, Kagawa, 769-2193, Japan.
Faculty of Science and Engineering, Tokushima Bunri University, 1314-1 Shido, Sanuki, Kagawa, 769-2193, Japan.
出版信息
Genes Environ. 2022 Feb 15;44(1):7. doi: 10.1186/s41021-022-00236-3.
Urea (Ua) is produced in DNA as the result of oxidative damage to thymine and guanine. It was previously reported that Klenow fragment (Kf) exo incorporated dATP opposite Ua, and that DNA polymerase β was blocked by Ua. We report here the following nucleotide incorporations opposite Ua by various DNA polymerases: DNA polymerase α, dATP and dGTP (dATP > dGTP); DNA polymerase δ, dATP; DNA polymerase ζ, dATP; Kf exo, dATP; Sulfolobus solfataricus P2 DNA polymerase IV (Dpo4), dGTP and dATP (dGTP > dATP); and DNA polymerase η, dCTP, dGTP, dATP, and dTTP (dCTP > dGTP > dATP > dTTP). DNA polymerases β and ε were blocked by Ua. Elongation by DNA polymerases δ and ζ stopped after inserting dATP opposite Ua. Importantly, the elongation efficiency to full-length beyond Ua using DNA polymerase η and Dpo4 were almost the same as that of natural DNA.
尿素（Ua）是DNA中胸腺嘧啶和鸟嘌呤发生氧化损伤的产物。此前有报道称，klenow片段（Kf）外切酶在Ua对面掺入dATP，并且DNA聚合酶β会被Ua阻断。我们在此报告各种DNA聚合酶在Ua对面的以下核苷酸掺入情况：DNA聚合酶α，dATP和dGTP（dATP > dGTP）；DNA聚合酶δ，dATP；DNA聚合酶ζ，dATP；Kf外切酶，dATP；嗜热栖热菌P2 DNA聚合酶IV（Dpo4），dGTP和dATP（dGTP > dATP）；以及DNA聚合酶η，dCTP、dGTP、dATP和dTTP（dCTP > dGTP > dATP > dTTP）。DNA聚合酶β和ε被Ua阻断。DNA聚合酶δ和ζ在Ua对面插入dATP后停止延伸。重要的是，使用DNA聚合酶η和Dpo4延伸至Ua以外全长的效率与天然DNA几乎相同。
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