Kino Katuhito, Ito Nobutoshi, Sugasawa Kaoru, Sugiyama Hiroshi, Hanaoka Fumio
Cellular Physiology Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Nucleic Acids Symp Ser (Oxf). 2004(48):171-2. doi: 10.1093/nass/48.1.171.
Guanine is the most oxidizable base among natural bases. 8-Oxoguanine (8-oxoG) is the typical oxidative product, but the amount of 8-oxoG does not directly reflect the strength of oxidative stress. Imidazolone, oxazolone and guanidinohydantoin are oxidative products of guanine and 8-oxoG. Here, we investigated enzymatic reactions with human DNA polymerase eta on these lesions.
鸟嘌呤是天然碱基中最易被氧化的碱基。8-氧代鸟嘌呤（8-oxoG）是典型的氧化产物，但8-oxoG的量并不能直接反映氧化应激的强度。咪唑酮、恶唑酮和胍基乙内酰脲是鸟嘌呤和8-oxoG的氧化产物。在此，我们研究了人类DNA聚合酶η对这些损伤的酶促反应。
