Porteous Nuala, Sun Yuyu, Schoolfield John
Gen Dent. 2015 Jan-Feb;63(1):41-7.
Previous studies have found inconsistent results from testing methods used to measure heterotrophic plate count (HPC) bacteria in dental unit waterline (DUWL) samples. This study used 63 samples to compare the results obtained from an in-office chairside method and 2 currently used commercial laboratory HPC methods (Standard Methods 9215C and 9215E). The results suggest that the Standard Method 9215E is not suitable for application to DUWL quality monitoring, due to the detection of limited numbers of heterotrophic organisms at the required 35°C incubation temperature. The results also confirm that while the in-office chairside method is useful for DUWL quality monitoring, the Standard Method 9215C provided the most accurate results.
先前的研究发现,用于测量牙科设备水线(DUWL)样本中异养平板计数(HPC)细菌的检测方法结果不一致。本研究使用63个样本,比较了在诊室内椅旁方法与2种目前使用的商业实验室HPC方法(标准方法9215C和9215E)所获得的结果。结果表明,由于在所需的35°C培养温度下检测到的异养生物数量有限,标准方法9215E不适用于DUWL质量监测。结果还证实,虽然诊室内椅旁方法对DUWL质量监测有用,但标准方法9215C提供了最准确的结果。