Porteous Nuala, Luo Jie, Hererra Monica, Schoolfield John, Sun Yuyu
Department of Comprehensive Dentistry, The University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA.
Int J Microbiol. 2011;2011:767314. doi: 10.1155/2011/767314. Epub 2011 Dec 15.
This study examined bacterial growth and type on biofilm-controlling dental unit waterline (DUWL) tubing (T) and control manufacturer's tubing (C) in a laboratory DUWL model using ultrapure source water that was cycled through the lines. Sections of tubing lines were detached and examined for biofilm growth using SEM imaging at six sampling periods. Bacteria from inside surfaces of T and C, source unit, and reservoir were cultured and enumerated. At six months, organisms were molecularly identified from the alignment matches obtained from the top three BLAST searches for the 16S region. There was a 1-3 log increase in organism growth in a clean, nonsterile reservoir within an hour. Biofilm was established on the inside surfaces of C within three weeks, but not on T. Proteobacteria, and Sphingomonas spp. were identified in the source reservoir and C line, and a variation of the genera was found in T line.
本研究在实验室牙科设备水线（DUWL）模型中，使用循环通过管路的超纯水，检测了生物膜控制型牙科设备水线（DUWL）管材（T）和对照制造商管材（C）上的细菌生长情况及类型。在六个采样期，分离出管路部分，使用扫描电子显微镜（SEM）成像检查生物膜生长情况。对T和C的内表面、水源单元和储水箱中的细菌进行培养和计数。在六个月时，从16S区域的前三次BLAST搜索获得的比对匹配中对生物进行分子鉴定。在清洁的非无菌储水箱中，一小时内生物体生长增加了1至3个对数级。三周内C的内表面形成了生物膜，但T上没有。在水源储水箱和C管路中鉴定出变形菌门和鞘氨醇单胞菌属，在T管路中发现了该属的变异。
