Institute of Biomedical Engineering, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, PR China.
State Key Laboratory of New Ceramics and Fine Processing, Department of Material Science and Engineering, Tsinghua University, Beijing 100084, PR China.
Colloids Surf B Biointerfaces. 2015 Feb 1;126:188-97. doi: 10.1016/j.colsurfb.2014.12.019. Epub 2014 Dec 30.
The migration of vascular endothelial cells (ECs) is essential for reendothelialization after implantation of cardiovascular biomaterials. Reendothelialization is largely determined by surface properties of implants. In this study, surfaces modified with various chemical functional groups (CH3, NH2, COOH, OH) prepared by self-assembled monolayers (SAMs) were used as model system. Expressions and distributions of critical proteins in the integrin-induced signaling pathway were examined to explore the mechanisms of surface chemistry regulating EC migration. The results showed that SAMs modulated cell migration were in the order CH3>NH2>OH>COOH, determined by differences in the expressions of focal adhesion components and Rho GTPases. Multiple integrin subunits showed difference in a surface chemistry-dependent manner, which induced a stepwise activation of signaling cascades associated with EC migration. This work provides a broad overview of surface chemistry regulated endothelial cell migration and establishes association among the surface chemistry, cell migration behavior and associated integrin signaling events. Understanding the relationship between these factors will help us to understand the surface/interface behavior between biomaterials and cells, reveal molecular mechanism of cells sensing surface characterization, and guide surface modification of cardiovascular implanted materials.
血管内皮细胞（ECs）的迁移对于心血管生物材料植入后的再内皮化至关重要。再内皮化在很大程度上取决于植入物的表面特性。在本研究中，使用通过自组装单分子层（SAM）制备的具有各种化学官能团（CH3、NH2、COOH、OH）的表面作为模型系统。研究了整合素诱导的信号通路中关键蛋白的表达和分布，以探讨表面化学调节 EC 迁移的机制。结果表明，SAM 调节细胞迁移的顺序为 CH3>NH2>OH>COOH，这是由粘着斑成分和 Rho GTPases 的表达差异决定的。多种整合素亚基以依赖于表面化学的方式表现出差异，这诱导了与 EC 迁移相关的信号级联的逐步激活。这项工作提供了一个广泛的表面化学调节内皮细胞迁移的概述，并建立了表面化学、细胞迁移行为和相关整合素信号事件之间的联系。了解这些因素之间的关系将有助于我们了解生物材料和细胞之间的表面/界面行为，揭示细胞感知表面特征的分子机制，并指导心血管植入材料的表面改性。
