Institute of Biomedical Engineering, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
ACS Appl Mater Interfaces. 2013 Jun 12;5(11):5112-21. doi: 10.1021/am400973a. Epub 2013 May 15.
Vascular endothelial cell (EC) adhesion and migration are essential processes in re-endothelialization of implanted biomaterials, which are affected by surface properties of implanted materials such as surface wettability. Our previous study demonstrated that, as model substrates, EC adhesion/migration showed an opposite behavior on the hydrophobic and hydrophilic surfaces of plasma SiOx:H nanocoatings. Extending our previous works, the expression and distribution of crucial proteins in integrins-FAK-Rho GTPases signaling pathways were examined, respectively. The results showed that a hydrophilic surface could enhance the expression of focal adhesion protein associated with cell adhesion; however, the hydrophobic surface could improve the expression of Rho GTPases associated with cell migration and phosphorylation level of FAK, revealing the potential reason of surface wettability mediating different cells' adhesion/migration behaviors. These findings reveal the relationship and molecular mechanism of endothelial cell adhesion/migration, which was expected to guide the surface modification of implants for accelerating endothelialization.
血管内皮细胞（EC）黏附和迁移是植入生物材料再内皮化的必要过程，其受到植入材料表面特性的影响，如表面润湿性。我们之前的研究表明，作为模型底物，EC 在等离子体 SiOx:H 纳米涂层的疏水和亲水表面上表现出相反的黏附/迁移行为。在我们之前工作的基础上，进一步研究了整合素-FAK-Rho GTPases 信号通路中关键蛋白的表达和分布。结果表明，亲水表面可以增强与细胞黏附相关的黏着斑蛋白的表达；然而，疏水表面可以提高与细胞迁移相关的 Rho GTPases 以及 FAK 的磷酸化水平，揭示了表面润湿性调节不同细胞黏附/迁移行为的潜在原因。这些发现揭示了内皮细胞黏附和迁移的关系和分子机制，有望指导植入物表面改性以加速内皮化。
