Wang Liang, Shao Jianli, Zhang Xia, Xu Meng, Zhao Jianfu
Department of Oncology, First Affiliated Hospital, Jinan University, Guangzhou, 510632, People's Republic of China,
Tumour Biol. 2015 May;36(5):3911-7. doi: 10.1007/s13277-014-3034-2. Epub 2015 Jan 11.
MicroRNAs (miRNAs) are essential to the progression of osteosarcoma. Previous research using osteosarcoma samples confirmed that miR-377 expression is less than that observed in normal human osteoblast expression. These data suggest a role for miR-377 in osteosarcoma that warrants investigation. To address this concept, we measured miR-377 expression in two cell models, and we also observed that miR-377 was less expressed in osteosarcoma MG-63 cells compared to the hFOB1.19 human fetal osteoblastic cell line. Moreover, miR-377 overexpression reduced cell proliferation and suppressed invasion of MG-63 cells but had no effect on MG-63 cell apoptosis. Because cyclin-dependent kinase 6 (CDK6) may be a potential target of miR-377 in osteosarcoma cells, we overexpressed CDK6 and observed that overexpression attenuated tumor suppressive effects of miR-377 on cell proliferation. Our data suggest that miR-377 can suppress proliferation in MG-63 cells in part by targeting CDK6.
微小RNA（miRNA）对骨肉瘤的进展至关重要。先前使用骨肉瘤样本的研究证实，miR-377的表达低于在正常人成骨细胞中的表达。这些数据表明miR-377在骨肉瘤中发挥作用，值得进一步研究。为了验证这一观点，我们在两种细胞模型中检测了miR-377的表达，并且我们还观察到与hFOB1.19人胎儿成骨细胞系相比，miR-377在骨肉瘤MG-63细胞中的表达较低。此外，miR-377的过表达降低了细胞增殖并抑制了MG-63细胞的侵袭，但对MG-63细胞凋亡没有影响。由于细胞周期蛋白依赖性激酶6（CDK6）可能是骨肉瘤细胞中miR-377的潜在靶点，我们过表达了CDK6，并观察到过表达减弱了miR-377对细胞增殖的肿瘤抑制作用。我们的数据表明，miR-377可以部分通过靶向CDK6来抑制MG-63细胞的增殖。
