School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
J Hazard Mater. 2015 Apr 9;286:171-8. doi: 10.1016/j.jhazmat.2015.01.001. Epub 2015 Jan 3.
Roxarsone (ROX) remains to be as an organoarsenical feed additive used widely in developing countries. However, most of the ROX is excreted unchanged in manure, which could be readily photodegraded into inorganic arsenic derivatives. In this study, the comparative cytotoxicity and arsenic accumulation were evaluated after the exposure of Tetrahymenathermophila (T. thermophila) cell model to ROX and its photodegradates. The cytotoxic effects were estimated according to the relevant cell growth curves, morphologies and MTT assays. The 36 h median effective concentrations for ROX and its photodegradates at various photolysis times (10, 20, and 30 min) are 39.0, 2.08, 1.88, and 1.82 mg (total arsenic) L(-1), respectively. In parallel, the cellular arsenic uptakes were determined by hydride generation-atomic fluorescence spectrometry. Phospholipid layer as basic membrane structure was mimicked to assess the correlation between membrane permeability and cytotoxicity. The biocompatibility of ROX was dependent on its tendency to interact with cell membrane while the cytotoxicity was induced by the trans-membrane of the inorganic arsenic species present in the photodegradates of ROX. Furthermore, the photodegradates of ROX-associated alterations of intracellular protein profiles were analyzed using a proteomic approach. Overall, the significance was clarified that the control of arsenic emission caused by the application of ROX needs to be imposed.
罗欧砷仍然是一种在发展中国家广泛使用的有机胂饲料添加剂。然而，大部分罗欧砷在粪便中以未改变的形式被排泄，这很容易在阳光下光解为无机砷衍生物。在这项研究中，通过暴露于 Tetrahymenathermophila (T. thermophila) 细胞模型，评估了罗欧砷及其光降解产物的比较细胞毒性和砷积累。根据相关细胞生长曲线、形态和 MTT 测定，评估了细胞毒性效应。在不同光解时间（10、20 和 30 分钟）下，罗欧砷及其光降解产物的 36 h 半数有效浓度分别为 39.0、2.08、1.88 和 1.82 mg（总砷）L(-1)。同时，通过氢化物发生-原子荧光光谱法测定细胞内砷的摄取量。以磷脂层作为基本膜结构来评估膜通透性与细胞毒性之间的相关性。罗欧砷的生物相容性取决于其与细胞膜相互作用的趋势，而毒性则是由罗欧砷光降解产物中存在的无机砷物种跨膜引起的。此外，还使用蛋白质组学方法分析了罗欧砷相关的细胞内蛋白质图谱的改变。总的来说，阐明了需要控制罗欧砷应用引起的砷排放的重要性。
