Liu Qingqing, Leslie Elaine M, Le X Chris
Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, 10-102 Clinical Sciences Building, University of Alberta , Edmonton, Alberta T6G 2G3, Canada.
Department of Physiology, Faculty of Medicine and Dentistry, 7-08A Medical Sciences Building, University of Alberta , Edmonton, Alberta T6G 2H7, Canada.
J Agric Food Chem. 2016 Nov 23;64(46):8902-8908. doi: 10.1021/acs.jafc.6b03341. Epub 2016 Nov 8.
Roxarsone (Rox), an organoarsenic compound, served as a feed additive in the poultry industry for more than 60 years. Residual amounts of Rox present in chicken meat could give rise to potential human exposure to Rox. However, studies on the bioavailability of Rox in humans are scarce. We report here the accumulation and transepithelial transport of Rox using the human colon-derived adenocarcinoma cell line (Caco-2) model. The cellular accumulation and transepithelial passage of Rox in Caco-2 cells were evaluated and compared to those of arsenobetaine (AsB), arsenite (As), and arsenate (As). When Caco-2 cells were exposed to 3 μM Rox, AsB, and As separately for 24 h, the maximum accumulation was reached at 12 h. After 24-h exposure, the accumulated Rox was 6-20 times less than AsB and As. The permeability of Rox from the apical to basolateral side of Caco-2 monolayers was similar to As but less than As and AsB. The results of lower bioavailability of Rox are consistent with previous observations of relatively lower amounts of Rox retained in the breast meat of Rox-fed chickens. These data provide useful information for assessing human exposure to and intestinal bioavailability of Roxarsone.
洛克沙胂（Rox）是一种有机砷化合物，在禽类养殖业中作为饲料添加剂已使用了60多年。鸡肉中残留的洛克沙胂可能会使人类潜在接触到该物质。然而，关于洛克沙胂在人体中的生物利用度的研究却很匮乏。我们在此报告利用人结肠源腺癌细胞系（Caco-2）模型对洛克沙胂的蓄积及跨上皮转运情况。评估了洛克沙胂在Caco-2细胞中的细胞蓄积和跨上皮转运，并与砷甜菜碱（AsB）、亚砷酸盐（As）和砷酸盐（As）进行了比较。当Caco-2细胞分别暴露于3 μM的洛克沙胂、砷甜菜碱和亚砷酸盐24小时时，12小时时达到最大蓄积量。暴露24小时后，洛克沙胂的蓄积量比砷甜菜碱和亚砷酸盐少6至20倍。洛克沙胂从Caco-2单层细胞的顶端向基底外侧的渗透率与亚砷酸盐相似，但低于砷酸盐和砷甜菜碱。洛克沙胂生物利用度较低的结果与之前观察到的喂食洛克沙胂的鸡胸脯肉中洛克沙胂残留量相对较低的情况一致。这些数据为评估人类对洛克沙胂接触情况及肠道生物利用度提供了有用信息。
