Institute of Hydrogeology and Environmental Geology, Chinese Academy of Geological Sciences, Shijiazhuang, China.
Key Laboratory of Groundwater Remediation of Hebei Province and China Geological Survey, Shijiazhuang, China.
Microbiologyopen. 2020 Apr;9(4):e1003. doi: 10.1002/mbo3.1003. Epub 2020 Feb 13.
The degradation of roxarsone, an extensively used organoarsenic feed additive, occurs quickly under anaerobic conditions with microorganisms playing an important role in its degradation. Here, an anaerobic bacterial consortium that effectively degraded roxarsone was isolated, and its degradation efficiency and community changes along a roxarsone concentration gradient under anaerobic conditions were assessed. We used batch experiments to determine the roxarsone degradation rates, as well as the bacterial community structure and diversity, at initial roxarsone concentrations of 50, 100, 200, and 400 mg/kg. The results showed that roxarsone was degraded completely within 28, 28, 36, and 44 hr at concentrations of 50, 100, 200, and 400 mg/kg, respectively. The anaerobic bacterial consortium displayed considerable potential to degrade roxarsone, as the degradation rate increased with increasing roxarsone concentrations. Roxarsone promoted microbial growth, and in turn, the microorganisms degraded the organoarsenic compound, with the functional bacterial community varying between different roxarsone concentrations. Lysinibacillus, Alkaliphilus, and Proteiniclasticum were the main genera composing the roxarsone-degrading bacterial community.
罗沙砷是一种广泛使用的有机胂饲料添加剂，在厌氧条件下迅速降解，微生物在其降解过程中发挥着重要作用。本研究分离出了一种能够有效降解罗沙砷的厌氧细菌混合菌群，并评估了其在厌氧条件下沿罗沙砷浓度梯度的降解效率和群落变化。我们采用批式实验，在初始罗沙砷浓度为 50、100、200 和 400mg/kg 条件下，测定了罗沙砷的降解速率以及细菌群落结构和多样性。结果表明，在 50、100、200 和 400mg/kg 浓度下，分别在 28、28、36 和 44hr 内完全降解了罗沙砷。厌氧细菌混合菌群对罗沙砷具有很强的降解潜力，随着罗沙砷浓度的增加，降解速率也随之增加。罗沙砷促进了微生物的生长，而微生物则降解了有机胂化合物，不同罗沙砷浓度下的功能细菌群落也有所不同。赖氨芽孢杆菌、嗜堿菌和蛋白菌是构成罗沙砷降解细菌群落的主要属。
