Ivanova Karina, Zehtindjiev Pavel, Mariaux Jean, Georgiev Boyko B
Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 2 Gagarin Street, 1113 Sofia, Bulgaria.
Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 2 Gagarin Street, 1113 Sofia, Bulgaria.
Infect Genet Evol. 2015 Apr;31:33-9. doi: 10.1016/j.meegid.2015.01.004. Epub 2015 Jan 8.
The knowledge of the diversity of haemosporidian parasites is of primary importance as their representatives include agents of bird malaria. We investigated the occurrence of Haemoproteus spp. and Plasmodium spp. in bird populations from a single locality in the State of Selangor, Peninsular Malaysia, and report on the parasite prevalence of the two genera. A combination of methods (molecular and morphological) was used for detecting these parasites. Seventy-nine bird individuals were caught using mist-nets in July and August 2010 at Gombak Field Station of the University of Malaya, Kuala Lumpur. In total, 23 birds were identified as positive for Haemoproteus or Plasmodium infection and one individual was recognized as carrying mixed infection. The total prevalence of haemosporidians in the collected samples was 30.3%. Infections with parasites of the genus Haemoproteus were predominant compared to those of the genus Plasmodium. In total, 10 new cyt b lineages of Haemoproteus spp. and 3 new cyt b lineages of Plasmodium spp. were recorded in this study. From all recorded haemosporidian lineages (16 in total), 3 were known from previous studies - hCOLL2, hYWT2 and pNILSUN1. Two of them are linked with their corresponding morphospecies - Haemoproteus pallidus (COLL2) and Haemoproteus motacillae (YWT2). The morphological analysis in the present study confirmed the results obtained by the PCR method relative to prevalence, with 25.3% total prevalence of Haemoproteus and Plasmodium parasites. The intensities of infection varied between 0.01% and 19%. Most infections were light, with intensities below 0.1%. The present study is the first molecular survey of the protozoan blood parasites of the order Haemosporida recorded in Malaysia.
了解血孢子虫寄生虫的多样性至关重要，因为它们的代表包括鸟类疟疾的病原体。我们调查了马来西亚半岛雪兰莪州一个地点鸟类种群中血变原虫属和疟原虫属的出现情况，并报告了这两个属的寄生虫患病率。采用多种方法（分子和形态学）检测这些寄生虫。2010年7月和8月，在吉隆坡马来亚大学的贡巴克野外站，用雾网捕获了79只鸟类个体。总共23只鸟被鉴定为血变原虫或疟原虫感染阳性，1只个体被认为携带混合感染。所采集样本中血孢子虫的总患病率为30.3%。与疟原虫属相比，血变原虫属寄生虫的感染更为普遍。本研究共记录了10个血变原虫属新的细胞色素b谱系和3个疟原虫属新的细胞色素b谱系。在所有记录的血孢子虫谱系（共16个）中，有3个谱系是先前研究已知的——hCOLL2、hYWT2和pNILSUN1。其中两个与它们相应的形态种相关——苍白血变原虫（COLL2）和鹡鸰血变原虫（YWT2）。本研究中的形态学分析证实了PCR方法在患病率方面获得的结果，血变原虫和疟原虫寄生虫的总患病率为25.3%。感染强度在0.01%至19%之间变化。大多数感染较轻，强度低于0.1%。本研究是马来西亚首次对血孢子虫目原生动物血液寄生虫进行的分子调查。
