外源24-表油菜素内酯对菠菜叶片中蜕皮甾体含量的影响
The effect of exogenous 24-epibrassinolide on the ecdysteroid content in the leaves of Spinacia oleracea L.
作者信息
Kamlar Marek, Rothova Olga, Salajkova Sarka, Tarkowska Dana, Drasar Pavel, Kocova Marie, Harmatha Juraj, Hola Dana, Kohout Ladislav, Macek Tomas
机构信息
Dept. of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, 16628, Czech Republic.
Dept. of Genetics and Microbiology, Faculty of Science, Charles University in Prague, 12843, Czech Republic.
出版信息
Steroids. 2015 May;97:107-12. doi: 10.1016/j.steroids.2014.12.024. Epub 2015 Jan 9.
The aim of this study was to show whether/how the application of exogenous 24-epibrassinolide can affect the content of ecdysteroids in spinach leaves. Brassinosteroids and ecdysteroids, structurally related phytosterols, show effect on a range of processes in plants. Brassinosteroids increase biomass yield in some species, photosynthesis and resistance to stress, and ecdysteroids show effect on proteins responsible for binding of CO2 or water cleavage. The mutual interaction of these sterols in plants is unclear. The UPLC-(+)ESI-MS/MS analyses of extracts of treated and untreated spinach (Spinacia oleracea L.) leaves show that the application of exogenous 24-epibrassinolide does influence the ecdysteroid content in plant tissues. The response differs for the major ecdysteroids and also differs from that for the minor ones and is dependent on the developmental stage of the leaves within the same plant or the 24-epibrassinolide concentration applied.
本研究的目的是表明外源24-表油菜素内酯的应用是否/如何影响菠菜叶片中蜕皮甾体的含量。油菜素甾体和蜕皮甾体是结构相关的植物甾醇，对植物的一系列生理过程有影响。油菜素甾体可提高某些物种的生物量产量、光合作用和抗逆性，而蜕皮甾体对负责二氧化碳结合或水裂解的蛋白质有影响。这些甾醇在植物中的相互作用尚不清楚。对处理过和未处理过的菠菜（Spinacia oleracea L.）叶片提取物进行的超高效液相色谱-（+）电喷雾串联质谱分析表明，外源24-表油菜素内酯的应用确实会影响植物组织中蜕皮甾体的含量。主要蜕皮甾体的反应不同，次要蜕皮甾体的反应也不同，并且取决于同一植株内叶片的发育阶段或所施用的24-表油菜素内酯浓度。
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