Derevyanchuk Michael, Kretynin Sergii, Iakovenko Oksana, Litvinovskaya Raisa, Zhabinskii Vladimir, Martinec Jan, Blume Yaroslav, Khripach Vladimir, Kravets Volodymyr
Department of the Molecular Mechanisms of Cell Metabolism Regulation, Institute of Bioorganic Chemistry and Petrochemistry, The National Academy of Sciences of Ukraine, 02660, Murmanska str., 1, Kyiv, Ukraine.
Laboratory of Steroid Chemistry, Institute of Bioorganic Chemistry, The National Academy of Sciences of Belarus, 220141, Kuprevich str., 5, Minsk, Belarus.
Steroids. 2017 Jan;117:16-24. doi: 10.1016/j.steroids.2016.11.006. Epub 2016 Nov 29.
Using Brassica napus roots we observed statistically significant increase in alternative respiratory pathway in response to exogenous 24-epibrassinolide (EBL) under optimal conditions and salinity. Also we observed activation of phospholipid signaling under the same conditions in response to EBL by measuring levels of lipid second messengers - diacylglycerol (DAG) and phosphatidic acid (PA). We found that brassinosteroids cause closure of stomata in isolated leaf disks while inhibitors of alternative oxidase cancelled these effects. This study demonstrates that BRs activate total respiration rate, alternative respiratory pathway, production of PA and DAG, stimulate stomata closure and growth under optimal conditions and salinity. Also, specific inhibitor of brassinosteroids biosynthesis decreased alternative respiratory pathway and production of lipid messengers in rape plants.
使用甘蓝型油菜根，我们观察到在最佳条件和盐度下，外源性24-表油菜素内酯（EBL）可使交替呼吸途径有统计学意义的增加。同样，在相同条件下，通过测量脂质第二信使——二酰基甘油（DAG）和磷脂酸（PA）的水平，我们观察到EBL可激活磷脂信号传导。我们发现油菜素甾醇可导致离体叶盘气孔关闭，而交替氧化酶抑制剂可消除这些作用。本研究表明，油菜素甾醇在最佳条件和盐度下可激活总呼吸速率、交替呼吸途径、PA和DAG的产生，刺激气孔关闭和生长。此外，油菜素甾醇生物合成的特异性抑制剂可降低油菜植株的交替呼吸途径和脂质信使的产生。
