从耳叶牛皮消根中提取的抗癫痫C21甾体类化合物。

Antiepileptic C21 steroids from the roots of Cynanchum otophyllum.

作者信息

Li Xiang, Zhang Mi, Xiang Cheng, Li Bao-Cai, Li Peng

机构信息

a Faculty of Life Science and Technology, Kunming University of Science and Technology , Kunming 650500 , China.

出版信息

J Asian Nat Prod Res. 2015;17(7):724-32. doi: 10.1080/10286020.2014.1001380. Epub 2015 Jan 12.

DOI:10.1080/10286020.2014.1001380

PMID:25579837

Abstract

In order to discover more natural products possessing potentially antiepileptic activities, three C21 steroids, including a new one, characterized as caudatin-3-O-β-D-cymaropyranosyl-(1 → 4)-β-D-cymaropyranosyl-(1 → 4)-β-D-cymaropyranoside (1), and two known analogs, otophylloside B (2) and caudatin-3-O-β-D-oleandropyranosyl-(1 → 4)-β-D-oleandropyranosyl-(1 → 4)-β-D-cymaropyranosyl-(1 → 4)-β-D-cymaropyranoside (3), were isolated from the chloroform extract of the roots of Cynanchum otophyllum and evaluated for their antiepileptic activities by pentylenetrazole (PTZ)-induced zebrafish larval locomotor assay. The results showed that all of them had marked activities of suppressing PTZ-induced seizure behaviors in larval zebrafish at the dose of 10 μg/ml.

摘要

为了发现更多具有潜在抗癫痫活性的天然产物，从耳叶牛皮消根的氯仿提取物中分离出三种C21甾体化合物，其中包括一种新的化合物，鉴定为考达汀-3-O-β-D- cymaropyranosyl-(1→4)-β-D-cymaropyranosyl-(1→4)-β-D-cymaropyranoside(1)，以及两种已知类似物，耳叶牛皮消苷B(2)和考达汀-3-O-β-D- oleandropyranosyl-(1→4)-β-D-oleandropyranosyl-(1→4)-β-D-cymaropyranosyl-(1→4)-β-D-cymaropyranoside(3)，并通过戊四氮(PTZ)诱导的斑马鱼幼体运动试验评估它们的抗癫痫活性。结果表明，在10μg/ml的剂量下，它们均具有显著的抑制斑马鱼幼体PTZ诱导的癫痫行为的活性。