Jarvis M F, Williams M
Research Department, Ciba-Geigy Corporation, Summit, NJ 07901.
Eur J Pharmacol. 1989 Sep 13;168(2):243-6. doi: 10.1016/0014-2999(89)90571-2.
The regional distribution of adenosine A2 receptors in the rat brain was determined using the A2-selective agonist ligand 3Hphenylamino)-5'-N-carboxamidoadenosine (CGS 21680) by quantitative receptor autoradiography. [3H]CGS 21680 binding was highly localized in the striatal region of the rat brain with the greatest density of binding found in the caudate-putamen, nucleus accumbens and olfactory tubercle. Additionally, lower levels of binding were also found in the globus pallidus. No significant amounts of [3H]CGS 21,680 binding were detected in other brain regions. This localization of brain adenosine A2 receptors was markedly different from the known regional distribution of A1 receptors which are highly concentrated in cerebellum, hippocampus, thalamus and cortex. The present results provide further evidence for a specific contribution of adenosine in the modulation of central neurotransmission.
通过定量受体放射自显影术，使用A2选择性激动剂配体3H苯基氨基)-5'-N-羧酰胺基腺苷（CGS 21680）来确定大鼠脑中腺苷A2受体的区域分布。[3H]CGS 21680结合高度定位于大鼠脑的纹状体区域，在尾状核-壳核、伏隔核和嗅结节中发现结合密度最高。此外，苍白球中也发现了较低水平的结合。在其他脑区未检测到大量的[3H]CGS 21680结合。脑腺苷A2受体的这种定位与已知的A1受体区域分布明显不同，A1受体高度集中在小脑海马、丘脑和皮层。目前的结果为腺苷在调节中枢神经传递中的特定作用提供了进一步的证据。
