Almeida J, Carpenter R, Robbins T P, Martin C, Coen E S
John Innes Institute, Norwich, UK.
Genes Dev. 1989 Nov;3(11):1758-67. doi: 10.1101/gad.3.11.1758.
Diverse spatial patterns of flower color in Antirrhinum can be produced by a series of alleles of pallida, a gene encoding an enzyme required for pigment biosynthesis. The alleles arose by imprecise excision of a transposable element, Tam3, and we show that they carry a series of deletions involving progressive removal of sequences adjacent to the excision site. This has enabled us to define three cis-acting upstream regions, A, B, and C, which differentially affect the level of pallida expression in distinct areas of the flower. We show further that an unlinked locus, delila, regulates the spatial distribution of pallida transcript. Deletion of regions ABC at the pallida locus uncouples pallida from regulation by delila, whereas deletion of A or AB brings pallida under regulation by delila in a new area of the flower. These results suggest that diverse patterns of pallida expression reflect the different ways in which alleles interact with a prepattern of both common and spatially specific genetic signals in the flower.
金鱼草中多样的花色空间模式可由pallida基因的一系列等位基因产生，该基因编码色素生物合成所需的一种酶。这些等位基因是由转座元件Tam3的不精确切除产生的，我们发现它们携带一系列缺失，涉及与切除位点相邻序列的逐步去除。这使我们能够定义三个顺式作用上游区域，A、B和C，它们在花的不同区域差异地影响pallida的表达水平。我们进一步表明，一个不连锁的基因座delila调节pallida转录本的空间分布。在pallida基因座上删除区域ABC会使pallida与delila的调控脱钩，而删除A或AB会使pallida在花的一个新区域受到delila的调控。这些结果表明，pallida表达的多样模式反映了等位基因与花中常见和空间特异性遗传信号的预模式相互作用的不同方式。
