A Mutation in the Gene Is Associated with Purple Pigmentation in .

Anthocyanins are well-known antioxidants that are beneficial for plants and consumers. Dihydroflavonol-4-reductase () is a key gene of anthocyanin biosynthesis, controlled by multiple transcription factors. Its expression can be enhanced by mutations in the negative regulator of anthocyanin biosynthesis myeloblastosis family transcription factor-like 2 (). The expression profiles of the gene were examined in 43 purple and green varieties of L., L., L., and L. gene expression was significantly reduced in purple varieties of , and green varieties of . The gene sequences were screened for mutations that can affect pigmentation. Expression of the gene was cultivar-specific, but in general it correlated with anthocyanin content and was higher in purple plants. Two single nucleotide polymorphysms (SNPs) were found at the beginning of the DNA-binding domain of gene in all purple varieties of . This mutation, leading to an amino acid substitution and the formation of a mononucleotide repeat (A), significantly affects RNA structure. No other noteworthy mutations were found in the gene in green varieties of and other studied species. These results bring new insights into the regulation of anthocyanin biosynthesis in genus and provide opportunities for generation of new purple varieties with precise mutations introduced via genetic engineering and CRISPR/Cas.