Colburn N H, Raab-Traub N, Becker D, Cao Y, Winterstein D
Laboratory of Viral Carcinogenesis, National Cancer Institute, Frederick, MD 21701.
Int J Cancer. 1989 Dec 15;44(6):1012-6. doi: 10.1002/ijc.2910440613.
A JB6 mouse epidermal recipient cell line has been used to detect nasopharyngeal carcinoma (NPC) DNA-associated transforming activity that is not detectable in the NIH 3T3 focus assay. NPC DNA showed both transforming activity and activity for transferring sensitivity to tumor-promoter-induced neoplastic transformation, assayed in 2 different variants of mouse JB6 cells. Comparison of DNAs from various NPC sources that did or did not harbor EBV DNA and that varied in degree of differentiation showed similar transforming activities and similar activities for transferring promotion sensitivity. Thus both a NPC DNA-associated promotion sensitivity and an oncogenic activity function independently of concurrent EBV gene expression.
一种JB6小鼠表皮受体细胞系已被用于检测鼻咽癌（NPC）DNA相关的转化活性，而这种活性在NIH 3T3焦点试验中是检测不到的。在小鼠JB6细胞的2种不同变体中进行检测，NPC DNA既表现出转化活性，又表现出传递对肿瘤启动子诱导的肿瘤转化敏感性的活性。对来自各种鼻咽癌来源的DNA进行比较，这些DNA有的含有EBV DNA，有的不含有EBV DNA，且分化程度不同，结果显示它们具有相似的转化活性和相似的传递促进敏感性的活性。因此，与NPC DNA相关的促进敏感性和致癌活性均独立于EBV基因的同时表达发挥作用。
