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1
A transforming activity not detected by DNA transfer to NIH 3T3 cells is detected by JB6 mouse epidermal cells.一种未通过将DNA转移至NIH 3T3细胞检测到的转化活性，可被JB6小鼠表皮细胞检测到。
Mol Cell Biol. 1985 Apr;5(4):890-3. doi: 10.1128/mcb.5.4.890-893.1985.
2
Transfer of sensitivity to tumor promoters by transfection of DNA from sensitive into insensitive mouse JB6 epidermal cells.通过将敏感小鼠JB6表皮细胞的DNA转染到不敏感细胞中，使细胞对肿瘤启动子产生敏感性转移。
Mol Cell Biol. 1983 Jul;3(7):1182-6. doi: 10.1128/mcb.3.7.1182-1186.1983.
3
Transfer by pro gene transfection of tumor promoter-sensitive phenotype to promotion-insensitive JB6 cells.
Cancer Res. 1988 Mar 1;48(5):1195-200.
4
Four-day duration of tumor promoter exposure required to transform JB6 promotion-sensitive cells to anchorage independence.将JB6促癌敏感细胞转化为不依赖贴壁生长状态所需的肿瘤启动子暴露持续时间为四天。
Cancer Res. 1988 Dec 15;48(24 Pt 1):7126-31.
5
Blocking of tumor promoter-induced AP-1 activity inhibits induced transformation in JB6 mouse epidermal cells.阻断肿瘤启动子诱导的AP-1活性可抑制JB6小鼠表皮细胞的诱导转化。
Proc Natl Acad Sci U S A. 1994 Jan 18;91(2):609-13. doi: 10.1073/pnas.91.2.609.
6
Synergistic inhibition of phorbol ester-induced transformation of JB6 cells by transforming growth factor-beta and retinoic acid.转化生长因子-β和视黄酸对佛波酯诱导的JB6细胞转化的协同抑制作用。
Cancer Res. 1991 Feb 15;51(4):1158-64.
7
Transforming activity of nasopharyngeal carcinoma DNA detectable in mouse JB6 cells.在小鼠JB6细胞中可检测到的鼻咽癌DNA的转化活性。
Int J Cancer. 1989 Dec 15;44(6):1012-6. doi: 10.1002/ijc.2910440613.
8
Inhibition by tumor-promoting phorbol esters of procollagen synthesis in promotable JB6 mouse epidermal cells.促癌佛波酯对可促癌的JB6小鼠表皮细胞中前胶原合成的抑制作用。
J Natl Cancer Inst. 1982 Nov;69(5):1147-54.
9
Carba-prostacyclin inhibits 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced transformation in sensitive murine epidermal JB6 cells.卡巴前列环素可抑制12-O-十四酰佛波醇-13-乙酸酯（TPA）诱导的敏感小鼠表皮JB6细胞发生转化。
Carcinogenesis. 1992 Oct;13(10):1859-62. doi: 10.1093/carcin/13.10.1859.
10
Tumor-promoter-resistant cells lack trisialoganglioside response.对肿瘤促进剂有抗性的细胞缺乏三唾液酸神经节苷脂反应。
Proc Natl Acad Sci U S A. 1982 Aug;79(16):4988-91. doi: 10.1073/pnas.79.16.4988.

引用本文的文献

1
Differential expression of an 80-kDa protein kinase C substrate in preneoplastic and neoplastic mouse JB6 cells.
Proc Natl Acad Sci U S A. 1989 Oct;86(19):7410-4. doi: 10.1073/pnas.86.19.7410.

本文引用的文献

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Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase.筛选表达编码黄嘌呤 - 鸟嘌呤磷酸核糖转移酶的大肠杆菌基因的动物细胞。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2072-6. doi: 10.1073/pnas.78.4.2072.
2
Dissociation of mitogenesis and late-stage promotion of tumor cell phenotype by phorbol esters: mitogen-resistant variants are sensitive to promotion.佛波酯对肿瘤细胞表型的促有丝分裂作用与晚期促进作用的解离：有丝分裂抗性变体对促进作用敏感。
Proc Natl Acad Sci U S A. 1981 Nov;78(11):6912-6. doi: 10.1073/pnas.78.11.6912.
3
Retinoids inhibit promoter-dependent preneoplastic progression in mouse epidermal cell lines.维甲酸可抑制小鼠表皮细胞系中启动子依赖性的肿瘤前病变进展。
Ann N Y Acad Sci. 1981 Feb 27;359:251-9. doi: 10.1111/j.1749-6632.1981.tb12751.x.
4
Transfer of sensitivity to tumor promoters by transfection of DNA from sensitive into insensitive mouse JB6 epidermal cells.通过将敏感小鼠JB6表皮细胞的DNA转染到不敏感细胞中，使细胞对肿瘤启动子产生敏感性转移。
Mol Cell Biol. 1983 Jul;3(7):1182-6. doi: 10.1128/mcb.3.7.1182-1186.1983.
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Fibroblast immortality is a prerequisite for transformation by EJ c-Ha-ras oncogene.成纤维细胞永生化是EJ c-Ha-ras癌基因转化的前提条件。
Nature. 1983;304(5927):648-51. doi: 10.1038/304648a0.
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Immortalization and neoplastic transformation of normal diploid cells by defined cloned DNA fragments of herpes simplex virus type 2.单纯疱疹病毒2型特定克隆DNA片段使正常二倍体细胞永生化及肿瘤转化
Proc Natl Acad Sci U S A. 1983 Oct;80(19):5902-6. doi: 10.1073/pnas.80.19.5902.
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Expression of the large T protein of polyoma virus promotes the establishment in culture of "normal" rodent fibroblast cell lines.多瘤病毒大T蛋白的表达促进了“正常”啮齿动物成纤维细胞系在培养中的建立。
Proc Natl Acad Sci U S A. 1983 Jul;80(14):4354-8. doi: 10.1073/pnas.80.14.4354.
8
Tumorigenic conversion of primary embryo fibroblasts requires at least two cooperating oncogenes.原代胚胎成纤维细胞的致瘤性转化至少需要两个协同作用的癌基因。
Nature. 1983;304(5927):596-602. doi: 10.1038/304596a0.
9
Specific binding of transforming growth factor correlates with promotion of anchorage independence in EGF receptorless mouse JB6 cells.转化生长因子的特异性结合与无表皮生长因子受体的小鼠JB6细胞中锚定非依赖性的促进相关。
Biochem Biophys Res Commun. 1981 Sep 30;102(2):799-807. doi: 10.1016/s0006-291x(81)80202-1.
10
Dual evolutionary origin for the rat genetic sequences of Harvey murine sarcoma virus.哈维鼠肉瘤病毒大鼠基因序列的双重进化起源
J Virol. 1980 Nov;36(2):408-20. doi: 10.1128/JVI.36.2.408-420.1980.

一种未通过将DNA转移至NIH 3T3细胞检测到的转化活性，可被JB6小鼠表皮细胞检测到。

A transforming activity not detected by DNA transfer to NIH 3T3 cells is detected by JB6 mouse epidermal cells.

作者信息

Colburn N H, Lerman M I, Hegamyer G A, Gindhart T D

出版信息

Mol Cell Biol. 1985 Apr;5(4):890-3. doi: 10.1128/mcb.5.4.890-893.1985.

DOI:10.1128/mcb.5.4.890-893.1985
PMID:2985973
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC366795/
Abstract

Transfection of four different mouse epidermal tumor cell DNAs into NIH 3T3 cells yielded neither morphologically altered foci nor anchorage independence. However, promotion-sensitive, but not promotion-insensitive, JB6 mouse epidermal cell lines were permissive for the expression of anchorage independence after transfection of DNA from three of these tumor cell lines. This transforming activity and the promotion-sensitive activity that confers sensitivity to promotion of transformation show differences in restriction enzyme sensitivity. In view of this difference and the differences in both recipient cells and 12-O-tetradecanoyl-phorbol-13-acetate dependence of expression, it appears that the transforming activity and the promotion-sensitive activity are specified by different genes. The JB6 promotion-sensitive cell lines may be useful for detecting and cloning transforming genes that escape detection in the NIH 3T3 cell focus assay.

摘要

将四种不同的小鼠表皮肿瘤细胞DNA转染到NIH 3T3细胞中，既未产生形态改变的集落，也未产生不依赖贴壁生长的特性。然而，对促癌敏感（而非促癌不敏感）的JB6小鼠表皮细胞系，在转染其中三种肿瘤细胞系的DNA后，能够表达不依赖贴壁生长的特性。这种转化活性以及赋予转化对促癌敏感性的促癌敏感活性，在限制酶敏感性方面存在差异。鉴于这种差异以及在受体细胞和12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯表达依赖性方面的差异，似乎转化活性和促癌敏感活性是由不同基因所决定的。JB6促癌敏感细胞系可能有助于检测和克隆那些在NIH 3T3细胞集落形成试验中无法检测到的转化基因。