Abrahamsen T G, Kjeldsberg E, Mortensson-Egnund K, Anestad G
Tidsskr Nor Laegeforen. 1989 Nov 30;109(33):3419-20.
Electron microscopy, ELISA for rotavirus and virus cultivation in cell cultures were used to study the occurrence of rotavirus in 451 fecal samples collected from hospitalized children. Rotavirus was found in samples from 24 of 27 patients admitted with acute diarrhoea. All specimens from randomly selected patients without diarrhoea were negative. Two patients developed nosocomial rotavirus infection. ELISA was positive, but electron microscopy was negative in two specimens obtained from asymptomatic newborns. In this investigation rotavirus was the dominating agent isolated in infectious gastroenteritis. We found no rotavirus excretors without diarrhoea.
采用电子显微镜、轮状病毒酶联免疫吸附测定(ELISA)以及细胞培养中的病毒培养法,对从住院儿童采集的451份粪便样本进行研究,以了解轮状病毒的感染情况。在27例因急性腹泻入院的患者中,有24例的样本检测出轮状病毒。随机选取的无腹泻症状患者的所有样本均为阴性。有两名患者发生了医院内轮状病毒感染。从无症状新生儿获取的两份样本,ELISA检测呈阳性,但电子显微镜检测呈阴性。在本次调查中,轮状病毒是感染性肠胃炎中分离出的主要病原体。我们未发现无腹泻症状的轮状病毒排泄者。