Krida G, Rhim A, Daaboub J, Failloux A-B, Bouattour A
Département de Protection des Plantes et de Maladies Post-Récolte, Institut National Agronomique de Tunisie, University of Carthage, Tunis, Tunisia; Laboratoire d'Épidémiologie et de Microbiologie Vétérinaire, Institut Pasteur de Tunis, University of Tunis El Manar, Tunis, Tunisia.
Med Vet Entomol. 2015 Jun;29(2):124-8. doi: 10.1111/mve.12107. Epub 2015 Jan 14.
Physiological and molecular characteristics of natural populations of Culex pipiens Linnaeus, 1758 (Diptera: Culicidae) were investigated to elucidate how this species is potentially involved in the transmission of West Nile virus in Tunisia. A total of 215 Cx. pipiens females from 11 breeding habitats were analysed in the laboratory to estimate autogeny and stenogamy rates. They were tested individually for the locus CQ11 to distinguish between the two Cx. pipiens forms, pipiens and molestus. All tested Cx. pipiens populations were stenogamous. Females from underground breeding sites were all autogeneous, whereas females from above-ground habitats were mostly anautogeneous. Of all the females tested, 59.7% were identified as pipiens, 22.4% as molestus, and 17.9% as hybrid pipiens/molestus. Furthermore, both Cx. pipiens forms and their hybrids were found to co-occur in sympatry in all sites. The results of this study represent the first evidence that both Cx. pipiens forms and their hybrids are present in Tunisia. Because hybrids able to act as bridge vectors are present in all studied habitats, Tunisia can be considered to have a high degree of receptivity for the establishment of West Nile virus zoonotic cycles.
对尖音库蚊（Culex pipiens Linnaeus，1758）（双翅目：蚊科）自然种群的生理和分子特征进行了研究，以阐明该物种在突尼斯如何潜在地参与西尼罗河病毒的传播。在实验室中对来自11个繁殖栖息地的总共215只尖音库蚊雌蚊进行了分析，以估计自体繁殖和近亲繁殖率。对它们分别进行CQ11位点测试，以区分尖音库蚊的两种类型，即尖音库蚊指名亚种（pipiens）和骚扰库蚊（molestus）。所有测试的尖音库蚊种群都是近亲繁殖的。来自地下繁殖地点的雌蚊都是自体繁殖的，而来自地上栖息地的雌蚊大多是非自体繁殖的。在所有测试的雌蚊中，59.7%被鉴定为尖音库蚊指名亚种，22.4%为骚扰库蚊，17.9%为尖音库蚊指名亚种/骚扰库蚊杂交种。此外，在所有地点都发现尖音库蚊的两种类型及其杂交种在同域共存。这项研究的结果首次证明突尼斯存在尖音库蚊的两种类型及其杂交种。由于在所有研究栖息地都存在能够充当桥梁媒介的杂交种，突尼斯可被认为对建立西尼罗河病毒人畜共患病循环具有高度易感性。
