Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, IA 50011, USA.
Biosensors (Basel). 2013 Sep 13;3(3):312-26. doi: 10.3390/bios3030312.
Anisotropic nanoparticles (i.e., silver nanocubes) were functionalized with target-specific antibodies and Raman active tags to serve as nanoprobes for the rapid detection of bacteria in a test-in-a-tube platform. A self-referencing scheme was developed and implemented in which surface enhanced Raman spectroscopic (SERS) signatures of the targets were observed superimposed with the SERS signals of the Raman tags. The assessment through the dual signals (superimposed target and tag Raman signatures) supported a specific recognition of the targets in a single step with no washing/separation needed to a sensitivity of 102 CFU/mL, even in the presence of non-target bacteria at a 10 times higher concentration. The self-referencing protocol implemented with a portable Raman spectrometer potentially can become an easy-to-use, field-deployable spectroscopic sensor for onsite detection of pathogenic microorganisms.
各向异性纳米粒子（即银纳米立方体）经靶向特异性抗体和拉曼活性标记物功能化，用作纳米探针，用于在管中测试平台上快速检测细菌。开发并实施了一种自参照方案，其中观察到目标的表面增强拉曼光谱（SERS）特征与拉曼标记物的 SERS 信号叠加。通过双重信号（叠加的目标和标记物拉曼特征）进行评估，支持在无需洗涤/分离的情况下，在单个步骤中对目标进行特异性识别，灵敏度达到 102 CFU/mL，即使在非目标细菌浓度高 10 倍的情况下也是如此。使用便携式拉曼光谱仪实施的自参照方案可能成为一种易于使用、可现场部署的光谱传感器，用于现场检测致病性微生物。
