Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, IA 50011, USA.
Biosensors (Basel). 2020 Dec 4;10(12):200. doi: 10.3390/bios10120200.
Among the physical, chemical and biological hazards that could arise with respect to food safety, bacterial contamination has been one of the main concerns in recent years. Bacterial contamination in low moisture foods (LMFs) was an emerging threat which used to draw less attention as LMFs were considered at low risk of such a hazard. Bacteria can survive in low moisture environments and cause foodborne diseases once they enter the digestive system. Common detection methods such as ELISA and PCR are not well suited to LMFs, as most of them operate under aqueous environments. In this study, a Dual Immunological Raman-Enabled Crosschecking Test (DIRECT) was developed for LMFs using a nano-scaled surface enhanced Raman scattering (SERS) biosensor platform and multivariate discriminant analysis with a portable Raman spectrometer. It could provide a limit of detection (LOD) of 10 CFU/g of bacteria in model LMFs, with a detection time of 30-45 min. It has the potential to become a quick screening method for on-site bacteria detection for LMFs to identify food safety risks in real time.
在食品安全方面可能出现的物理、化学和生物危害中，细菌污染近年来一直是主要关注点之一。低水分食品（LMF）中的细菌污染是一种新出现的威胁，由于 LMF 被认为风险较低，因此过去对此关注较少。细菌可以在低水分环境中存活，并在进入消化系统后引起食源性疾病。ELISA 和 PCR 等常见检测方法并不适用于 LMF，因为它们大多数都在水相环境下操作。在这项研究中，使用纳米级表面增强拉曼散射（SERS）生物传感器平台和带有便携式拉曼光谱仪的多元判别分析，开发了一种用于 LMF 的双免疫拉曼增强交叉检查测试（DIRECT）。它可以在模型 LMF 中提供 10 CFU/g 细菌的检测限（LOD），检测时间为 30-45 分钟。它有可能成为 LMF 现场细菌检测的快速筛选方法，以便实时识别食品安全风险。
