Dai Jian-Ying, Zhao Pan, Cheng Xiao-Long, Xiu Zhi-Long
School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, People's Republic of China,
Appl Biochem Biotechnol. 2015 Mar;175(6):3014-24. doi: 10.1007/s12010-015-1481-x. Epub 2015 Jan 14.
2,3-Butanediol has been known as a platform green chemical, and the production cost is the key problem for its large-scale production in which the carbon source occupies a major part. Sugarcane molasses is a by-product of sugar industry and considered as a cheap carbon source for biorefinery. In this paper, the fermentation of 2,3-butanediol with sugarcane molasses was studied by reducing the medium ingredients and operation steps. The fermentation medium was optimized by response surface methodology, and 2,3-butanediol production was explored under the deficiency of sterilization, molasses acidification, and organic nitrogen source. Based on these experiments, the fermentation medium with sugarcane molasses as carbon source was simplified to five ingredients, and the steps of molasses acidification and medium sterilization were reduced; thus, the cost was reduced and the production of 2,3-butanediol was enhanced. Under fed-batch fermentation, 99.5 g/L of 2,3-butanediol and acetoin was obtained at 60 h with a yield of 0.39 g/g sugar.
2,3-丁二醇是一种公认的平台绿色化学品，其生产成本是大规模生产的关键问题，其中碳源占主要部分。甘蔗废蜜是制糖工业的副产品，被认为是生物炼制的廉价碳源。本文通过减少培养基成分和操作步骤，研究了以甘蔗废蜜发酵生产2,3-丁二醇的过程。采用响应面法对发酵培养基进行优化，并在灭菌不足、废蜜酸化和有机氮源缺乏的条件下探索2,3-丁二醇的生产情况。基于这些实验，以甘蔗废蜜为碳源的发酵培养基简化为五种成分，减少了废蜜酸化和培养基灭菌步骤；从而降低了成本，提高了2,3-丁二醇的产量。在分批补料发酵条件下，60 h时获得了99.5 g/L的2,3-丁二醇和乙偶姻，糖产率为0.39 g/g。
