Deshmukh Apoorva Nandkumar, Nipanikar-Gokhale Padmaja, Jain Rishi
Department of Technology, Savitribai Phule Pune University, Ganeshkhind Road, Pune, 411 007, Maharashtra, India.
Praj Matrix R & D Center, Division of Praj Industries Ltd., 402/403/1098, Urawade, Pirangut, Mulshi, Pune, 412 115, Maharashtra, India.
Appl Biochem Biotechnol. 2016 May;179(2):321-31. doi: 10.1007/s12010-016-1996-9. Epub 2016 Jan 29.
2,3-butanediol is known to be a platform chemical with several potential industrial applications. Sustainable industrial scale production can be attained by using a sugarcane molasses based fermentation process using Bacillus subtilis. However, the accumulation of acetoin needs to be reduced to improve process efficiency. In this work, B. subtilis was genetically modified in order to increase the yield of 2,3-butanediol. Metabolic engineering strategies such as cofactor engineering and overexpression of the key enzyme butanediol dehydrogenase were attempted. Both the strategies individually led to a statistically significant increase in the 2,3-butanediol yields for sugarcane molasses based fermentation. Cofactor engineering led to a 26 % increase in 2,3-butanediol yield and overexpression of bdhA led to a 11 % increase. However, the combination of the two strategies did not lead to a synergistic increase in 2,3-butanediol yield.
2,3-丁二醇是一种具有多种潜在工业应用的平台化学品。通过使用基于甘蔗废蜜的发酵工艺，利用枯草芽孢杆菌可实现可持续的工业规模生产。然而，为了提高工艺效率，需要减少乙偶姻的积累。在这项工作中，对枯草芽孢杆菌进行了基因改造，以提高2,3-丁二醇的产量。尝试了代谢工程策略，如辅因子工程和关键酶丁二醇脱氢酶的过表达。这两种策略单独使用时，均使基于甘蔗废蜜的发酵中2,3-丁二醇的产量有统计学意义的显著增加。辅因子工程使2,3-丁二醇产量提高了26%，bdhA的过表达使产量提高了11%。然而，这两种策略的组合并未使2,3-丁二醇产量产生协同增加。
