利用作物生物质利用菌解淀粉芽孢杆菌亚种 SDM 从木薯粉生产高效 2,3-丁二醇。
Efficient 2,3-butanediol production from cassava powder by a crop-biomass-utilizer, Enterobacter cloacae subsp. dissolvens SDM.
机构信息
State Key Laboratory of Microbial Technology, Shandong University, Jinan, People's Republic of China.
出版信息
PLoS One. 2012;7(7):e40442. doi: 10.1371/journal.pone.0040442. Epub 2012 Jul 5.
BACKGROUND
2,3-Butanediol (BD) is considered as one of the key platform chemicals used in a variety of industrial applications. It is crucial to find an efficient sugar-utilizing strain and feasible carbon source for the economical production of BD.
METHODOLOGY/PRINCIPAL FINDINGS: Efficient BD production by a newly isolated Enterobacter cloacae subsp. dissolvens SDM was studied using crop-biomass cassava powder as substrate. The culture conditions and fermentation medium for BD production were optimized. Under the optimal conditions, 78.3 g l(-1) of BD was produced after 24 h in simultaneous saccharification and fermentation (SSF), with a yield of 0.42 g BD g(-1) cassava powder and a specific productivity of 3.3 g l(-1) h(-1). A higher BD concentration (93.9 g l(-1)) was produced after 47 h in fed-batch SSF.
CONCLUSIONS/SIGNIFICANCE: The results suggest that strain SDM is a good candidate for the BD production, and cassava powder could be used as an alternative substrate for the efficient production of BD.
背景
2,3-丁二醇（BD）被认为是在各种工业应用中使用的关键平台化学品之一。找到一种高效的糖利用菌株和可行的碳源，对于经济生产 BD 至关重要。
方法/主要发现：使用作物生物质木薯粉作为底物，研究了新分离的阴沟肠杆菌亚种 dissolvens SDM 对 BD 的高效生产。优化了 BD 生产的培养条件和发酵培养基。在最佳条件下，在同步糖化发酵（SSF）24 小时后，可生产 78.3 g/L 的 BD，木薯粉的产率为 0.42 g BD/g，比生产率为 3.3 g/L/h。在补料分批 SSF 中，47 小时后可生产出更高浓度的 BD（93.9 g/L）。
结论/意义：结果表明，SDM 菌株是生产 BD 的良好候选菌株，木薯粉可用作高效生产 BD 的替代底物。
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