Medical Device Development Center , Daegu-Gyeongbuk Medical Innovation Foundation, Daegu 701-310, South Korea.
Center for BioMicrosystems, Korea Institute of Science and Technology , Seoul 136-791, South Korea.
Biomicrofluidics. 2014 Dec 5;8(6):066504. doi: 10.1063/1.4902943. eCollection 2014 Nov.
Cell encapsulation technology is a promising strategy applicable to tissue engineering and cell therapy. Many advanced microencapsulation chips that function via multiple syringe pumps have been developed to generate mono-disperse hydrogel beads encapsulating cells. However, their operation is difficult and only trained microfluidic engineers can use them with dexterity. Hence, we propose a microfluidic manifold system, driven by a single syringe pump, which can enable the setup of automated flow sequences and generate highly mono-disperse alginate beads by minimizing disturbances to the pump pressure. The encapsulation of P19 mouse embryonic carcinoma cells and embryonic body formation are demonstrated to prove the efficiency of the proposed system.
细胞封装技术是一种有前途的策略，适用于组织工程和细胞治疗。已经开发出许多通过多个注射器泵运行的先进微封装芯片，以生成包封细胞的单分散水凝胶珠。然而，它们的操作很困难，只有经过训练的微流控工程师才能熟练使用。因此，我们提出了一种由单个注射器泵驱动的微流控歧管系统，可以通过最小化对泵压的干扰来实现自动流序列的设置，并生成高度单分散的海藻酸钠珠。通过封装 P19 小鼠胚胎癌细胞和胚胎体形成来证明所提出系统的效率。
