Center of Excellence for Functional Materials and Laboratory of Physical Chemistry, Department of Natural Sciences, Åbo Akademi University, Turku, Finland; E-Mails:
Center of Excellence for Functional Materials and Physics, Department of Natural Sciences, Åbo Akademi University, Turku, Finland; E-Mail:
Biosensors (Basel). 2012 Dec 27;3(1):1-17. doi: 10.3390/bios3010001. eCollection 2013 Mar.
In this article, we report on the formation and mode-of-operation of an affinity biosensor, where alternate layers of biotin/streptavidin/biotinylated-CRP-antigen/anti-CRP antibody are grown on printed gold electrodes on disposable paper-substrates. We have successfully demonstrated and detected the formation of consecutive layers of supra-molecular protein assembly using an electrical (impedimetric) technique. The formation process is also supplemented and verified using conventional surface plasmon resonance (SPR) measurements and surface sensitive characterization techniques, such as X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The article provides a possible biosensor development scheme, where-(1) fabrication of paper substrate (2) synthesis of gold nanoparticle inks (3) inkjet printing of gold electrodes on paper (4) formation of the biorecognition layers on the gold electrodes and (5) electrical (impedimetric) analysis of growth-all are coupled together to form a test-structure for a recyclable and inexpensive point-of-care diagnostic platform.
本文报告了一种亲和生物传感器的形成和工作模式，其中交替层的生物素/链霉亲和素/生物素化-CRP 抗原/抗 CRP 抗体在可重复使用的纸质基底上的印刷金电极上生长。我们已经成功地使用电气（阻抗）技术证明和检测了超分子蛋白质组装的连续层的形成。该形成过程也通过传统的表面等离子体共振（SPR）测量和表面敏感特性分析技术，如 X 射线光电子能谱（XPS）和原子力显微镜（AFM）得到了补充和验证。本文提供了一种可能的生物传感器开发方案，其中包括：（1）纸基的制备；（2）金纳米粒子油墨的合成；（3）在纸上喷墨打印金电极；（4）在金电极上形成生物识别层；（5）生长的电气（阻抗）分析——所有这些都结合在一起，形成了一个可回收和廉价的即时诊断平台的测试结构。
