Department of Analytical Chemistry, Faculty for Chemistry, University of Vienna, Währinger Straße 38, A-1090 Vienna, Austria; E-Mails:
Biosensors (Basel). 2014 Jun 16;4(2):161-71. doi: 10.3390/bios4020161. eCollection 2014 Jun.
Designing Molecularly Imprinted Polymers for sensing proteins is still a somewhat empirical process due to the inherent complexity of protein imprinting. Based on Bovine Serum Albumin as a model analyte, we explored the influence of a range of experimental parameters on the final sensor responses. The optimized polymer contains 70% cross linker. Lower amounts lead to higher sensitivity, but also sensor response times substantially increase (to up to 10 h) at constant imprinting effect (signal ratio MIP/NIP on quartz crystal microbalance-QCM). However, by shifting the polymer properties to more hydrophilic by replacing methacrylic acid by acrylic acid, part of the decreased sensitivity can be recovered leading to appreciable sensor responses. Changing polymer morphology by bulk imprinting and nanoparticle approaches has much lower influence on sensitivity.
由于蛋白质印迹的固有复杂性，设计用于检测蛋白质的分子印迹聚合物仍然是一个有些经验性的过程。以牛血清白蛋白（Bovine Serum Albumin）为模型分析物，我们探索了一系列实验参数对最终传感器响应的影响。优化后的聚合物含有 70%的交联剂。交联剂含量较低会导致更高的灵敏度，但也会使传感器响应时间大大延长（在恒定印迹效应（石英晶体微天平-QCM 上的 MIP/NIP 信号比）下，响应时间延长至 10 小时）。然而，通过用丙烯酸代替甲基丙烯酸使聚合物性质变得更亲水，部分恢复了灵敏度，从而产生了可观的传感器响应。通过本体印迹和纳米粒子方法改变聚合物形态对灵敏度的影响要小得多。
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