Muratsugu M, Ohta F, Miya Y, Hosokawa T, Kurosawa S, Kamo N, Ikeda H
Department of Laboratory Medicine, School of Medicine, Asahikawa Medical College, Japan.
Anal Chem. 1993 Oct 15;65(20):2933-7. doi: 10.1021/ac00068a036.
We have developed a piezoelectric immunosensor for the detection of microalbumin. Human serum albumin (HSA) in the range 0.1-100 micrograms mL-1 could be detected using a flow cell; the immunosensor is sensitive enough to monitor levels of albuminuria. The immunosensor did not respond to bovine serum albumin, only to HSA, implying that the specificity for HSA was high. We investigated the relationship between the frequency change (delta F) and adsorption per unit area of piezoelectrically active quartz crystal (delta M). delta M was estimated with radioisotope-labeled anti-HSA or HSA. When anti-HSA was adsorbed onto the surface of the crystal or HSA was bound to anti-HSA supported by the crystal, values of magnitude of delta F/delta M were larger than the value predicted from theory (Sauerbrey's equation). Furthermore, magnitude of delta F/delta M for HSA was larger than that for anti-HSA.
我们开发了一种用于检测微量白蛋白的压电免疫传感器。使用流通池可检测浓度范围为0.1 - 100微克/毫升的人血清白蛋白（HSA）；该免疫传感器灵敏度足以监测蛋白尿水平。该免疫传感器对牛血清白蛋白无反应，仅对HSA有反应，这意味着对HSA的特异性很高。我们研究了频率变化（δF）与压电活性石英晶体单位面积吸附量（δM）之间的关系。δM用放射性同位素标记的抗HSA或HSA进行估算。当抗HSA吸附到晶体表面或HSA与晶体支持的抗HSA结合时，δF/δM的大小值大于理论预测值（索末菲方程）。此外，HSA的δF/δM大小值大于抗HSA的该值。
