University of Vienna, Faculty for Chemistry, Department of Physical Chemistry, Währinger Straße 42, 1090, Vienna, Austria.
Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand.
Anal Bioanal Chem. 2018 Jan;410(3):875-883. doi: 10.1007/s00216-017-0442-3. Epub 2017 Jun 29.
Decreased blood level of high-density lipoprotein (HDL) is one of the essential criteria in diagnosing metabolic syndrome associated with the development of atherosclerosis and coronary heart disease. Herein, we report the synthesis of a molecularly imprinted polymer (MIP) that selectively binds HDL, namely, HDL-MIP, and thus serves as an artificial, biomimetic sensor layer. The optimized polymer contains methacrylic acid and N-vinylpyrrolidone in the ratio of 2:3, cross-linked with ethylene glycol dimethacrylate. On 10 MHz dual electrode quartz crystal microbalances (QCM), such HDL-MIP revealed dynamic detection range toward HDL standards in the clinically relevant ranges of 2-250 mg/dL HDL cholesterol (HDL-C) in 10 mM phosphate-buffered saline (PBS, pH = 7.4) without significant interference: low-density lipoprotein (LDL) yields 5% of the HDL signal, and both very-low-density lipoprotein (VLDL) and human serum albumin (HSA) yield 0%. The sensor reveals recovery rates between 94 and 104% at 95% confidence interval with precision of 2.3-7.7% and shows appreciable correlation (R = 0.97) with enzymatic colorimetric assay, the standard in clinical tests. In contrast to the latter, it achieves rapid results (10 min) during one-step analysis without the need for sample preparation. Graphical Abstract ᅟ.
高密度脂蛋白（HDL）水平降低是诊断与动脉粥样硬化和冠心病发展相关的代谢综合征的基本标准之一。在此，我们报告了一种选择性结合 HDL 的分子印迹聚合物（MIP）的合成，即 HDL-MIP，并将其用作人工、仿生传感器层。优化后的聚合物含有甲基丙烯酸和 N-乙烯基吡咯烷酮，摩尔比为 2:3，并与乙二醇二甲基丙烯酸酯交联。在 10 MHz 双电极石英晶体微天平（QCM）上，这种 HDL-MIP 在临床上相关的 2-250 mg/dL HDL 胆固醇（HDL-C）范围内，在 10 mM 磷酸盐缓冲盐水（PBS，pH = 7.4）中对 HDL 标准具有动态检测范围，且没有明显干扰：低密度脂蛋白（LDL）产生 5%的 HDL 信号，极低密度脂蛋白（VLDL）和人血清白蛋白（HSA）均产生 0%的 HDL 信号。该传感器在 95%置信区间内的回收率在 94%至 104%之间，精度为 2.3%至 7.7%，与临床测试中的标准酶比色法具有良好的相关性（R = 0.97）。与后者相比，它在一步分析中可实现快速（10 分钟）结果，而无需样品制备。
